FastQCFastQC Report
Thu 26 May 2016
ERR580273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5640820
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162680.28839778613747646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144850.2567889065774125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83340.1477444768668385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99700.054.6580241
GTATCAA187150.040.5489231
ATCAACG221000.033.071773
CAACGCA222150.032.773335
TATCAAC237650.031.5957662
GTGGTAT34400.030.753161
AACGCAG243500.030.4372836
TCAACGC244100.030.0188314
GTACTAG36850.028.4533251
ACGCAGA261100.027.82767
CGCAGAG264950.027.3347858
GTACATG225900.024.6850571
GCAGAGT289500.024.6754069
TGGTATC41350.024.560662
CTGTGCG44650.024.3141889
TACATGG229150.024.1295532
TAGGCAT46750.024.0287825
GCTATTG43750.023.7405748
TAGTACT51350.023.7069024
ACATGGG226150.023.4232233
Produced by FastQC (version 0.11.5)