##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580273_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5640820
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.549634273031224	34.0	31.0	34.0	31.0	34.0
2	32.666918639488586	34.0	31.0	34.0	31.0	34.0
3	32.71457819962346	34.0	31.0	34.0	31.0	34.0
4	36.23067213632061	37.0	35.0	37.0	35.0	37.0
5	36.18001141677983	37.0	35.0	37.0	35.0	37.0
6	36.21676352019742	37.0	37.0	37.0	35.0	37.0
7	36.16559808680298	37.0	36.0	37.0	35.0	37.0
8	36.16192291191706	37.0	37.0	37.0	35.0	37.0
9	37.8797958452849	39.0	38.0	39.0	35.0	39.0
10-11	37.83274577100492	39.0	38.0	39.0	35.0	39.0
12-13	37.7969461000351	39.0	38.0	39.0	35.0	39.0
14-15	39.17985860211813	41.0	39.0	41.0	36.0	41.0
16-17	39.17383075510298	40.0	39.0	41.0	36.0	41.0
18-19	39.17600003191025	40.0	39.0	41.0	36.0	41.0
20-21	39.150113724600324	40.0	39.0	41.0	36.0	41.0
22-23	39.07502792147241	40.0	39.0	41.0	35.0	41.0
24-25	39.00861089345166	40.0	38.0	41.0	35.0	41.0
26-27	38.88536923709673	40.0	38.0	41.0	35.0	41.0
28-29	38.78695836775504	40.0	38.0	41.0	35.0	41.0
30-31	38.62943898227563	40.0	38.0	41.0	35.0	41.0
32-33	38.513542711875225	40.0	38.0	41.0	34.0	41.0
34-35	38.416242673937475	40.0	38.0	41.0	34.0	41.0
36-37	38.297271584627765	40.0	38.0	41.0	34.0	41.0
38-39	38.178424945309374	40.0	38.0	41.0	34.0	41.0
40-41	38.06542789878067	40.0	37.5	41.0	33.5	41.0
42-43	38.06208317230474	40.0	37.5	41.0	33.0	41.0
44-45	38.01411878060282	40.0	37.5	41.0	33.0	41.0
46-47	37.82391593775373	40.0	37.0	41.0	33.0	41.0
48-49	37.61918019011421	40.0	37.0	41.0	33.0	41.0
50-51	37.43229229083715	40.0	36.5	41.0	32.5	41.0
52-53	37.27413691626394	40.0	36.0	41.0	32.0	41.0
54-55	37.0703516510011	39.0	35.5	41.0	32.0	41.0
56-57	36.8448283228325	39.0	35.0	41.0	31.0	41.0
58-59	36.61307593576821	39.0	35.0	41.0	31.0	41.0
60-61	36.37022596005545	38.5	35.0	40.5	31.0	41.0
62-63	36.07973663403548	38.0	35.0	40.0	30.5	41.0
64-65	35.794981580692166	37.0	35.0	40.0	30.0	41.0
66-67	35.49079743725203	37.0	35.0	40.0	29.5	41.0
68-69	35.18557018305849	36.0	34.0	39.0	29.0	41.0
70-71	34.874009541166004	36.0	34.0	39.0	29.0	41.0
72-73	34.564412354941304	35.0	34.0	39.0	29.0	40.0
74-75	34.2136887899277	35.0	34.0	37.5	29.0	40.0
76-77	32.67390627603788	34.5	31.5	36.0	26.5	39.0
78-79	33.553157785570185	35.0	33.5	37.0	28.0	39.0
80-81	33.52350323179964	35.0	34.0	36.0	29.0	39.0
82-83	33.2831290486135	35.0	33.5	36.0	28.5	37.0
84-85	33.05235745866736	35.0	33.0	35.5	28.0	37.0
86-87	32.84519112469464	35.0	33.0	35.0	27.0	37.0
88-89	32.65856985331919	35.0	33.0	35.0	27.0	36.0
90-91	32.48558862009424	35.0	33.0	35.0	27.0	36.0
92-93	32.322754404501474	35.0	33.0	35.0	26.0	36.0
94-95	32.18638983339302	35.0	33.0	35.0	25.5	35.0
96-97	32.06341755276715	35.0	32.5	35.0	25.0	35.0
98-99	31.96491059810453	35.0	32.0	35.0	25.0	35.0
100	30.134745302987863	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.03480272438433474
1101	2	-0.023012026224478177
1101	3	-0.01536691127147094
1101	4	-0.023605960034309703
1101	5	-0.03708648964121153
1101	6	-0.012453563993382488
1101	7	-0.03172525361138412
1101	8	-0.031043476259434044
1101	9	-0.08514769882103224
1101	10-11	-0.06016017107538829
1101	12-13	-0.0025306555392248242
1101	14-15	-0.02705251386998242
1101	16-17	-0.004310399346387328
1101	18-19	-0.025435931133465317
1101	20-21	0.025739259371746925
1101	22-23	-0.010661063706635332
1101	24-25	-0.04651314908594628
1101	26-27	-0.02308270844068261
1101	28-29	-0.0016528508588677937
1101	30-31	-0.06296700182245729
1101	32-33	-0.06949185408767988
1101	34-35	-0.0986919799563708
1101	36-37	-0.12312673365183713
1101	38-39	-0.14139575694589013
1101	40-41	-0.11377316728018627
1101	42-43	-0.022809732809569994
1101	44-45	-0.022148387399369085
1101	46-47	-0.04780715039360928
1101	48-49	-0.037869152465823674
1101	50-51	-0.05767892855630663
1101	52-53	-0.06924533169127045
1101	54-55	-0.01043039481089636
1101	56-57	-0.13233550994505094
1101	58-59	-0.13333706695677705
1101	60-61	-0.15422619864015275
1101	62-63	-0.09811897383574575
1101	64-65	-0.14655439800891656
1101	66-67	-0.11783293726397659
1101	68-69	-0.05992073346710214
1101	70-71	-0.1220551599264681
1101	72-73	-0.19875429103386466
1101	74-75	-0.16038523988398623
1101	76-77	-0.22840040503575665
1101	78-79	-0.06478069174927015
1101	80-81	-0.11047778958378984
1101	82-83	-0.12143025458329504
1101	84-85	-0.10846623834247282
1101	86-87	-0.17082284221524446
1101	88-89	-0.14559433116769327
1101	90-91	-0.11957924821989963
1101	92-93	-0.14606205860360433
1101	94-95	-0.2118140330248508
1101	96-97	-0.2700244641706142
1101	98-99	-0.2790366121091701
1101	100	-0.2881455866133287
1102	1	-0.12337967631331281
1102	2	-0.12278406517793883
1102	3	-0.097129897600162
1102	4	-0.07057892343343752
1102	5	-0.05056930012377592
1102	6	-0.05248092760756151
1102	7	-0.019345458924888703
1102	8	-0.028769871515940793
1102	9	-0.08330998304922588
1102	10-11	-0.068274340339876
1102	12-13	-0.10793212803686458
1102	14-15	-0.12178842138214918
1102	16-17	-0.10376908712705557
1102	18-19	-0.07122413418882445
1102	20-21	-0.0802017973831326
1102	22-23	-0.06251042741796908
1102	24-25	-0.13889017762826228
1102	26-27	-0.10397416267426252
1102	28-29	-0.0538932889155177
1102	30-31	-0.12885194827688196
1102	32-33	-0.10821560728313528
1102	34-35	-0.12583944083374377
1102	36-37	-0.11677009850397013
1102	38-39	-0.16296014423739535
1102	40-41	-0.17076378012617965
1102	42-43	-0.13355008132476343
1102	44-45	-0.07436280066124112
1102	46-47	1.049495811145107E-5
1102	48-49	-0.01947922819868353
1102	50-51	-0.04952020807430557
1102	52-53	-0.009724308029674944
1102	54-55	0.028398651683822607
1102	56-57	0.04560984971638504
1102	58-59	-0.09196298167475447
1102	60-61	-0.07734372180178894
1102	62-63	-0.039515280288696886
1102	64-65	0.009030833773493896
1102	66-67	-0.01974985832213605
1102	68-69	-0.014165684806179968
1102	70-71	-0.07876099970426509
1102	72-73	-0.13516377618299913
1102	74-75	-0.10973703810774538
1102	76-77	-0.09653276988147041
1102	78-79	-0.057941948156120304
1102	80-81	-0.07032302388719813
1102	82-83	-0.0011545757935991219
1102	84-85	-0.0575193309257358
1102	86-87	-0.14697429626548342
1102	88-89	-0.13836807802904616
1102	90-91	-0.07415463718609772
1102	92-93	-0.1308433135304483
1102	94-95	-0.030124105775918508
1102	96-97	-0.1585223372494795
1102	98-99	-0.2123363625696726
1102	100	0.021238894678617726
1103	1	0.00875336499311885
1103	2	0.021565295938799522
1103	3	0.012963397819348188
1103	4	0.017850692331414564
1103	5	-0.008330832670473853
1103	6	0.002338705136544661
1103	7	0.022046902267824464
1103	8	-0.03612223490218014
1103	9	-0.09161966813506695
1103	10-11	0.001877731293127738
1103	12-13	-0.043692229458777376
1103	14-15	0.007666896985647043
1103	16-17	0.04688286420785204
1103	18-19	0.04113867800935367
1103	20-21	0.022486845262712052
1103	22-23	0.006527967497284237
1103	24-25	-0.007606783276962403
1103	26-27	0.05153368872180408
1103	28-29	-0.01495851182721708
1103	30-31	-0.011948699927394557
1103	32-33	-0.03456671493724883
1103	34-35	-0.013266769788863542
1103	36-37	-0.08441851595205918
1103	38-39	-0.03049973407718909
1103	40-41	-0.04000916370193153
1103	42-43	0.04473646061845926
1103	44-45	0.012797057044572568
1103	46-47	0.012021745266984851
1103	48-49	0.007990129997921258
1103	50-51	-0.03352468433322997
1103	52-53	-0.10054076242743548
1103	54-55	-0.03181809424138038
1103	56-57	0.01245666760526376
1103	58-59	-0.05782505886237743
1103	60-61	-0.04869317422397046
1103	62-63	-0.03456342227417508
1103	64-65	-0.04271533927451543
1103	66-67	1.165168923478177E-4
1103	68-69	-0.06679294344002784
1103	70-71	-0.029351565316709127
1103	72-73	-0.02266340714683679
1103	74-75	-0.0561069076798546
1103	76-77	-0.023544071675793532
1103	78-79	-0.03089297474030417
1103	80-81	-0.09245207395853328
1103	82-83	-0.07189792630939706
1103	84-85	-0.05944510955268356
1103	86-87	-0.008629525462509946
1103	88-89	8.218582702710364E-4
1103	90-91	-0.0046495547842155815
1103	92-93	-0.05069488624709351
1103	94-95	-0.004588428625950769
1103	96-97	-0.012584812137490076
1103	98-99	-0.04682678461464462
1103	100	0.07736427399048651
1104	1	-0.03562498214758847
1104	2	-0.00987168080172296
1104	3	-0.05091496584495303
1104	4	-0.025642405786868494
1104	5	-0.05332912787102373
1104	6	-0.037644333279786224
1104	7	-0.027795048410602874
1104	8	-0.00635542732432981
1104	9	-0.011728755510965527
1104	10-11	-0.05648567706831642
1104	12-13	-0.041738965963403984
1104	14-15	-0.03455434620727971
1104	16-17	-0.03564069213637566
1104	18-19	-0.0684989896086492
1104	20-21	-0.09113027083139258
1104	22-23	-0.10452396796954133
1104	24-25	-0.06879119112646492
1104	26-27	-0.1398565331765269
1104	28-29	-0.08198358105315151
1104	30-31	-0.09915760187626432
1104	32-33	-0.08201203553156233
1104	34-35	0.03687135227741578
1104	36-37	-0.06269566390206904
1104	38-39	0.0205616973435383
1104	40-41	-0.05781966757521673
1104	42-43	0.020417953290582602
1104	44-45	-0.0416756718040574
1104	46-47	0.00821699833224443
1104	48-49	0.029993388410886723
1104	50-51	0.09320916332689677
1104	52-53	0.013608159406473419
1104	54-55	0.002603139043984015
1104	56-57	-0.04730537550501879
1104	58-59	-0.026346938045421098
1104	60-61	-0.08862591627432437
1104	62-63	-0.013705651228377747
1104	64-65	0.03170349363739433
1104	66-67	-0.002495117788356538
1104	68-69	-0.031121886686662492
1104	70-71	0.0020917273281284565
1104	72-73	-0.02050819961941386
1104	74-75	0.0014715145788883888
1104	76-77	0.03822779772831808
1104	78-79	0.09167898880541969
1104	80-81	0.04288345821098716
1104	82-83	0.07046564075769624
1104	84-85	0.08682280774439022
1104	86-87	0.08861228119350528
1104	88-89	0.14645988366858376
1104	90-91	0.04406574080571346
1104	92-93	0.05461110991745244
1104	94-95	0.1138079306163533
1104	96-97	0.02579446480987002
1104	98-99	0.017630646882860646
1104	100	0.19508369361857447
1105	1	-0.018790118672939116
1105	2	0.004648797709535302
1105	3	-0.007832303696233112
1105	4	-0.03107417061992379
1105	5	-0.039590816747875124
1105	6	-0.05914572872350021
1105	7	0.040574556597135825
1105	8	0.021127553478926586
1105	9	-0.024573300083218896
1105	10-11	-0.05263440293678201
1105	12-13	-0.004910268991331179
1105	14-15	0.007884414725381816
1105	16-17	-0.0022557701435417243
1105	18-19	-0.08211009715117967
1105	20-21	-0.030054319944888164
1105	22-23	-0.02759045732330634
1105	24-25	-0.04828432589633991
1105	26-27	-0.04557344035885791
1105	28-29	-0.12922918394880867
1105	30-31	-0.022519342387191443
1105	32-33	-0.038753118077181625
1105	34-35	-0.06178195077880844
1105	36-37	-0.0661714263157549
1105	38-39	-0.08259644866216576
1105	40-41	-0.0015280441694471847
1105	42-43	-0.03780644433670943
1105	44-45	-0.012025039471517118
1105	46-47	-0.04677212658886987
1105	48-49	-0.0065280864473109546
1105	50-51	-0.009765200917975392
1105	52-53	-0.06845099697392953
1105	54-55	-0.0402305985767768
1105	56-57	-0.03657654343308536
1105	58-59	-0.04433536054064291
1105	60-61	-0.04572904726669691
1105	62-63	-0.008045367644527346
1105	64-65	0.035529528190835435
1105	66-67	-0.08359565682528824
1105	68-69	-0.1040231963028333
1105	70-71	-0.030313717361444503
1105	72-73	-0.03732674496066579
1105	74-75	-0.028104858892127993
1105	76-77	-0.02398364219804705
1105	78-79	0.05166228656327121
1105	80-81	5.296943150980837E-4
1105	82-83	0.025478331269461307
1105	84-85	0.004767349708330926
1105	86-87	0.06930799426739043
1105	88-89	0.02350080202068483
1105	90-91	0.01308065499569011
1105	92-93	-0.048202798089278076
1105	94-95	-0.016024459120110635
1105	96-97	-0.004406799089586855
1105	98-99	-0.05620556525468956
1105	100	-0.22899909427384912
1106	1	0.04343016447629111
1106	2	-0.007642584782644235
1106	3	0.011606160082479278
1106	4	0.02308131480211273
1106	5	0.030449465450395508
1106	6	-0.010743012238265237
1106	7	-0.04075748253499256
1106	8	0.00935437152701013
1106	9	-0.018438474332327814
1106	10-11	0.01906467819522817
1106	12-13	0.01902000907619339
1106	14-15	0.012482562279181764
1106	16-17	0.01387484685313467
1106	18-19	-0.009412597738098327
1106	20-21	0.015321009548934228
1106	22-23	0.003839612721058927
1106	24-25	-0.004123981647730091
1106	26-27	-0.011722222234695323
1106	28-29	0.019613427217279877
1106	30-31	-0.06391576468607951
1106	32-33	-0.062175411027276084
1106	34-35	-0.10112600176231012
1106	36-37	-0.07987337296673758
1106	38-39	-0.08071801813743917
1106	40-41	-0.11763366124041852
1106	42-43	-0.10992407920257818
1106	44-45	-0.11510134905157798
1106	46-47	-0.09622489567426129
1106	48-49	-0.16037532612862293
1106	50-51	-0.13309720810369186
1106	52-53	-0.11744267901945449
1106	54-55	-0.22073684150027617
1106	56-57	-0.1848120798802313
1106	58-59	-0.21226056730080956
1106	60-61	-0.15131036426129896
1106	62-63	-0.20347541465775976
1106	64-65	-0.198215690991276
1106	66-67	-0.19973684214122756
1106	68-69	-0.1881028127081379
1106	70-71	-0.1421799339551555
1106	72-73	-0.1738992402875752
1106	74-75	-0.1848921901333398
1106	76-77	-0.1838135906974614
1106	78-79	-0.11921452732434545
1106	80-81	-0.1396927715913847
1106	82-83	-0.13950650532834885
1106	84-85	-0.11367128420359762
1106	86-87	-0.021490491472619055
1106	88-89	-0.07075755456666855
1106	90-91	-0.06305535574424681
1106	92-93	-0.031807230171210676
1106	94-95	-0.02548254765476088
1106	96-97	-0.09444079973147979
1106	98-99	-0.12479432873533725
1106	100	0.09354192343486645
1107	1	0.03199497902298276
1107	2	0.019583834664423705
1107	3	0.041585711280255566
1107	4	0.027799211099612364
1107	5	0.020869126802402604
1107	6	0.016875057031874974
1107	7	-0.013191778028627255
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2316	22-23	-0.13500745887355947
2316	24-25	-0.14805569485960746
2316	26-27	-0.15892240503600874
2316	28-29	-0.20828719953009056
2316	30-31	-0.15134996645367238
2316	32-33	-0.16138096777761035
2316	34-35	-0.2222773172149175
2316	36-37	-0.22522442061796966
2316	38-39	-0.20623678727525174
2316	40-41	-0.13937300039061995
2316	42-43	-0.1887184226027827
2316	44-45	-0.21988855449568234
2316	46-47	-0.17559017315333847
2316	48-49	-0.19083965170954542
2316	50-51	-0.0714539292523213
2316	52-53	-0.1524386338362902
2316	54-55	-0.21516223347340713
2316	56-57	-0.3286391431796645
2316	58-59	-0.31040746377483686
2316	60-61	-0.18402354285357347
2316	62-63	-0.11509616337507111
2316	64-65	-0.13732800370954124
2316	66-67	-0.2152403906373479
2316	68-69	-0.19382367458817384
2316	70-71	-0.16561607382632104
2316	72-73	-0.10088016392066379
2316	74-75	-0.07062568681014625
2316	76-77	-0.09390747479015715
2316	78-79	-0.10834981821910361
2316	80-81	-0.12880406021264434
2316	82-83	-0.14552371527846475
2316	84-85	-0.08880716907461306
2316	86-87	-0.06494708768141777
2316	88-89	-0.14490377769691776
2316	90-91	-0.14143173955532262
2316	92-93	-0.08819017076334035
2316	94-95	-0.10969692444053436
2316	96-97	-0.07478229767816913
2316	98-99	-0.06878838385223318
2316	100	-0.17697970037334088
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	3.0
13	35.0
14	203.0
15	726.0
16	1981.0
17	4601.0
18	8580.0
19	13349.0
20	19751.0
21	29728.0
22	38782.0
23	39070.0
24	35364.0
25	33924.0
26	38010.0
27	45927.0
28	56595.0
29	69819.0
30	86674.0
31	109589.0
32	138193.0
33	184552.0
34	278418.0
35	415974.0
36	715311.0
37	1336301.0
38	1521138.0
39	418049.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.511720983419096	21.080378156288642	13.953364270169889	23.454536590122373
2	15.124769260262012	21.080386009470946	40.80237577029255	22.99246895997449
3	17.881487390861146	27.39713690555334	30.478961131055264	24.24241457253025
4	12.249273546343675	17.907164620931333	39.572688710863204	30.27087312186179
5	12.649416269978458	38.67702045049597	35.34416580237392	13.329397477151645
6	29.259806198389597	38.78967951468049	18.318595523345895	13.631918763584016
7	25.70759412413449	33.214070634695375	23.63590850821936	17.44242673295077
8	25.302126734641377	35.80089890293844	20.49355802774646	18.403416334673718
9	26.17543933049943	15.46862097220543	21.550090395908907	36.80584930138623
10-11	24.697762633765226	26.54081468669418	28.505303275338377	20.25611940420222
12-13	25.29398004703752	24.408585175568113	28.590009974619807	21.707424802774565
14-15	22.760281199222906	25.642024550019283	26.47530625032631	25.1223880004315
16-17	21.557508562811783	28.28132239366411	27.40574879540932	22.755420248114792
18-19	21.15549418483028	27.818997568414495	29.73547554905683	21.290032697698397
20-21	22.45888155926584	27.00252100385964	29.31545185410921	21.22314558276531
22-23	22.574677602870842	26.743768608016644	28.966625501665217	21.7149282874473
24-25	22.08673492000177	26.909045782918938	29.40860701147897	21.595612285600318
26-27	21.938129964481327	27.14881765456207	28.959702660753685	21.95334972020292
28-29	21.44532434171688	27.44300020741702	29.268544765734	21.8431306851321
30-31	22.270078670919556	27.028928262956875	29.14653327452279	21.554459791600777
32-33	22.325734063769474	27.26123055319254	28.642157646173878	21.77087773686411
34-35	22.32602496802965	27.29588415489471	29.085957803482092	21.29213307359354
36-37	22.15796282412151	27.089243985517125	28.86127398307235	21.89151920728902
38-39	22.164698478264242	27.116901305452938	29.140448653883226	21.577951562399587
40-41	22.073282248951443	26.53664449291051	29.4630254004803	21.927047857657747
42-43	21.904657221445607	26.795196251459284	28.933915659606836	22.36623086748827
44-45	21.97664080360174	27.352925794307524	28.79885225077806	21.871581151312668
46-47	21.98394607739939	27.115847342805893	28.27374903103395	22.626457548760765
48-49	22.37985357174834	26.996430157588996	28.45456052465248	22.169155746010187
50-51	22.289288794415942	27.123010707278567	28.08057314217366	22.507127356131832
52-53	21.82934643986941	27.4939680025572	28.040058898569104	22.636626659004286
54-55	21.81619491107446	27.371244212899352	28.072140683853842	22.74042019217234
56-57	22.3693491165487	27.14001007156377	27.725582596351988	22.765058215535543
58-59	21.784861522620027	27.361648495662077	28.26871540192976	22.58477457978813
60-61	22.08452359793294	27.621936652289676	27.846735435704435	22.44680431407295
62-63	22.13406017969467	27.231156271997236	27.743457793274096	22.891325755033996
64-65	21.563770716504497	27.209405171379885	27.96682788650901	23.259996225606606
66-67	21.96298676196616	27.08975200969187	27.77261333827134	23.17464789007063
68-69	21.69221328233731	27.13704137685712	27.914825842140377	23.255919498665193
70-71	21.695388964308652	26.85318764096884	27.960612712802284	23.49081068192022
72-73	21.944595535673763	27.148898456292837	27.81596426383153	23.09054174420188
74-75	22.040358513834843	27.4040067399523	27.590314580749293	22.96532016546356
76-77	21.744931187829884	27.2834297656337	27.437350325463445	23.53428872107297
78-79	21.623374096506062	27.232094618521923	27.5962799819135	23.54825130305851
80-81	21.472518694081522	27.62597796120297	27.5990308985303	23.30247244618521
82-83	22.006282552836463	27.1075746894159	28.089909452842427	22.79623330490521
84-85	22.130503872880283	27.290476525203395	27.31974594990319	23.259273652013132
86-87	21.236673660584884	27.754181060043987	27.678790344669864	23.33035493470127
88-89	21.696982042125523	26.988019131026896	27.64445264661325	23.67054618023433
90-91	21.706469708812666	27.013971411162345	27.632445995937054	23.647112884087935
92-93	21.794880545197763	27.162943017725006	27.500270129381114	23.541906307696113
94-95	21.144161035560884	27.123046250353678	27.765201503645297	23.967591210440137
96-97	21.258953105287002	27.076190135976663	27.612195307665587	24.052661451070747
98-99	20.91563711776063	27.403758620989198	27.459939203001593	24.22066505824857
100	20.999085770731437	27.30125795607268	27.12082310062532	24.57883317257057
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	625.0
1	420.0
2	313.0
3	420.0
4	395.0
5	431.0
6	640.0
7	1207.5
8	1994.5
9	2562.0
10	2555.5
11	2385.0
12	2691.5
13	3120.0
14	3618.0
15	4264.5
16	4836.0
17	5558.0
18	6509.5
19	7418.0
20	8389.5
21	9572.5
22	11151.5
23	13136.5
24	15487.5
25	18654.0
26	22859.5
27	28035.5
28	34352.5
29	44213.5
30	57482.5
31	70417.0
32	86723.0
33	104502.5
34	121586.0
35	138615.0
36	157329.0
37	182655.0
38	194133.5
39	190744.5
40	185398.0
41	179661.0
42	179955.0
43	185132.0
44	194099.5
45	214003.0
46	230331.0
47	240230.5
48	246527.0
49	241619.5
50	232327.0
51	226511.5
52	218764.5
53	205988.0
54	194888.5
55	173455.5
56	151844.5
57	127505.0
58	102261.0
59	89158.5
60	71604.0
61	52518.0
62	39529.5
63	30779.5
64	22096.5
65	13059.0
66	8439.0
67	6027.0
68	4375.0
69	3214.0
70	2416.0
71	1784.0
72	1244.0
73	844.5
74	593.5
75	390.0
76	234.5
77	140.5
78	88.5
79	36.0
80	19.0
81	13.0
82	8.0
83	4.5
84	4.5
85	3.0
86	2.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005229736102197908
2	0.03352349481103811
3	0.0012409543293350968
4	0.0010814030584205842
5	0.006559330026485511
6	0.0
7	9.041238685155705E-4
8	0.006896160487305038
9	0.0013827776812591077
10-11	2.3932690637176865E-4
12-13	6.204771646675483E-5
14-15	8.952599090203197E-4
16-17	6.204771646675483E-5
18-19	9.927634634680773E-4
20-21	2.038710683907659E-4
22-23	0.0018791594129931464
24-25	0.0012409543293350968
26-27	0.0022159898738126726
28-29	1.7727918990501383E-4
30-31	0.0019146152509741492
32-33	0.001427097478735361
34-35	8.243482330583141E-4
36-37	0.004024237610843813
38-39	0.0010193553419538294
40-41	0.003864686339929301
42-43	0.003988781772862811
44-45	0.0038115025829577967
46-47	0.002526228456146447
48-49	0.009129878280108212
50-51	0.007170943231657808
52-53	0.00488404168188313
54-55	0.004573803099549356
56-57	0.003306256891728508
58-59	0.005885669104846458
60-61	0.007844604153296861
62-63	0.0034835360816335213
64-65	0.0031644335398044966
66-67	0.003838094461443549
68-69	0.0021007584003744136
70-71	0.0029694264309089815
72-73	0.006594785864466513
74-75	0.007516637651972586
76-77	0.004396523909644343
78-79	0.002632595970089455
80-81	0.0023223573877556806
82-83	0.004937225438854635
84-85	0.002153942157345918
86-87	0.0036342233930527833
88-89	6.29341124162799E-4
90-91	0.00154232895217362
92-93	7.977563545725622E-5
94-95	0.002269173630784177
96-97	0.0019412071294599013
98-99	0.0014359614382306119
100	5.3183756971504144E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5640820.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.67352069840357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94478300761676	45.604351053592126
2	16.031783954826082	19.774731191406012
3	4.968110610156725	9.192026176423775
4	2.017324104773484	4.976619197245436
5	1.0226753746442918	3.1535995442936184
6	0.5652482932403793	2.091651138773869
7	0.348469634095843	1.5043944433822563
8	0.22904562393365469	1.130084002284081
9	0.16329684903678796	0.9063982439148983
>10	0.6585793565303982	6.758390145560803
>50	0.03202427209508235	1.3613634508664632
>100	0.017100308950484255	1.9415427405170504
>500	0.0011335351730984012	0.4611377673659399
>1k	3.188061952291324E-4	0.289794566522162
>5k	3.542291058078617E-5	0.1820691984458772
>10k+	7.084582116157234E-5	0.671847139405575
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16268	0.28839778613747646	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14485	0.2567889065774125	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8334	0.1477444768668385	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.772791899050138E-5	0.0
12-13	0.0	0.0	0.0	1.772791899050138E-5	8.86395949525069E-6
14-15	0.0	0.0	0.0	1.772791899050138E-5	3.545583798100276E-5
16-17	0.0	0.0	0.0	1.772791899050138E-5	3.545583798100276E-5
18-19	0.0	0.0	0.0	4.431979747625346E-5	3.545583798100276E-5
20-21	0.0	0.0	0.0	7.091167596200553E-5	3.545583798100276E-5
22-23	0.0	0.0	0.0	1.2409543293350966E-4	4.431979747625346E-5
24-25	0.0	0.0	0.0	3.2796650132427556E-4	5.3183756971504144E-5
26-27	0.0	0.0	0.0	9.48443665991824E-4	7.091167596200553E-5
28-29	0.0	0.0	0.0	0.003199889377785499	7.091167596200553E-5
30-31	0.0	0.0	0.0	0.01347321843278105	7.97756354572562E-5
32-33	0.0	0.0	0.0	0.04128832332887772	8.86395949525069E-5
34-35	0.0	0.0	0.0	0.08940189546909846	8.86395949525069E-5
36-37	0.0	0.0	0.0	0.1675199705007428	8.86395949525069E-5
38-39	0.0	0.0	0.0	0.2784346956648147	8.86395949525069E-5
40-41	0.0	0.0	0.0	0.3992593275445768	8.86395949525069E-5
42-43	0.0	0.0	0.0	0.5238245503313348	8.86395949525069E-5
44-45	0.0	0.0	0.0	0.6793072638375273	8.86395949525069E-5
46-47	0.0	0.0	0.0	0.8646792487617049	8.86395949525069E-5
48-49	0.0	0.0	0.0	1.063435812523711	8.86395949525069E-5
50-51	0.0	0.0	0.0	1.2721997865558552	8.86395949525069E-5
52-53	0.0	0.0	0.0	1.4778525108051666	1.4182335192401105E-4
54-55	0.0	0.0	0.0	1.7064806180661676	1.950071088955152E-4
56-57	0.0	0.0	0.0	1.9741012831467768	1.950071088955152E-4
58-59	0.0	0.0	0.0	2.2781439577933704	1.950071088955152E-4
60-61	0.0	0.0	0.0	2.5969628529185473	1.950071088955152E-4
62-63	0.0	0.0	0.0	2.9170404302920496	2.1273502788601658E-4
64-65	0.0	0.0	0.0	3.243074588446361	2.1273502788601658E-4
66-67	0.0	0.0	0.0	3.6105211653624827	2.1273502788601658E-4
68-69	0.0	0.0	0.0	4.0229700646359925	2.1273502788601658E-4
70-71	0.0	0.0	0.0	4.470724114579086	2.1273502788601658E-4
72-73	0.0	0.0	0.0	4.920853705666906	2.6591878485752074E-4
74-75	0.0	0.0	0.0	5.3600274428185966	2.6591878485752074E-4
76-77	0.0	0.0	0.0	5.847403391705461	2.6591878485752074E-4
78-79	0.0	0.0	0.0	6.372796862867455	2.6591878485752074E-4
80-81	0.0	0.0	0.0	6.930712201417524	2.6591878485752074E-4
82-83	0.0	0.0	0.0	7.507126623434182	2.6591878485752074E-4
84-85	0.0	0.0	0.0	8.077885839292868	2.6591878485752074E-4
86-87	0.0	0.0	0.0	8.658989295882513	2.6591878485752074E-4
88	0.0	0.0	0.0	9.112983573310263	3.0137462283852346E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9970	0.0	54.658024	1
GTATCAA	18715	0.0	40.548923	1
ATCAACG	22100	0.0	33.07177	3
CAACGCA	22215	0.0	32.77333	5
TATCAAC	23765	0.0	31.595766	2
GTGGTAT	3440	0.0	30.75316	1
AACGCAG	24350	0.0	30.437283	6
TCAACGC	24410	0.0	30.018831	4
GTACTAG	3685	0.0	28.453325	1
ACGCAGA	26110	0.0	27.8276	7
CGCAGAG	26495	0.0	27.334785	8
GTACATG	22590	0.0	24.685057	1
GCAGAGT	28950	0.0	24.675406	9
TGGTATC	4135	0.0	24.56066	2
CTGTGCG	4465	0.0	24.314188	9
TACATGG	22915	0.0	24.129553	2
TAGGCAT	4675	0.0	24.028782	5
GCTATTG	4375	0.0	23.740574	8
TAGTACT	5135	0.0	23.706902	4
ACATGGG	22615	0.0	23.423223	3
>>END_MODULE