FastQCFastQC Report
Thu 26 May 2016
ERR580272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3927600
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90450.040.5488431
GGTATCA54400.039.933331
CAACGCA98600.035.2365655
ATCAACG105650.032.9771773
TATCAAC112250.032.4644362
TCAACGC112700.031.2472364
AACGCAG114050.031.230886
GTACTAG22400.029.8079991
ACGCAGA126750.027.100657
GTACATG185200.026.1764141
CGCAGAG133900.025.6181078
TACATGG187200.025.5702172
GCATCGT25400.024.2349628
ACATGGG195200.024.0389523
TAGGCAT34750.022.593725
CATGGGG165150.022.4052544
GCAGAGT149300.022.3181179
GTGGTAT21000.022.1670721
TGATCGC17800.021.3863269
CTAGGCA38050.021.1297874
Produced by FastQC (version 0.11.5)