FastQCFastQC Report
Thu 26 May 2016
ERR580272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3927600
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59400.038.1128961
GTATCAA92700.037.09071
CAACGCA101350.032.412625
ATCAACG106050.030.8435863
GTACTAG22900.030.276041
TATCAAC115150.029.5893082
AACGCAG115550.029.079426
TCAACGC114050.029.0100984
GTACATG182700.025.2216341
TACATGG187100.024.6659832
ACGCAGA133050.024.3715927
GTGGTAT23150.023.6332911
TAGGCAT32800.023.211435
ACATGGG191200.023.2033333
GTATAAG23300.023.0762981
CGCAGAG142300.022.7543288
TGATCGC16550.022.4328429
CTAGGCA36000.021.6709214
GTATAAC6350.021.539791
CATGGGG161200.021.1662244
Produced by FastQC (version 0.11.5)