FastQCFastQC Report
Thu 26 May 2016
ERR580271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7318352
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226920.31006980806607826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188500.2575716500108221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111450.15228838405149137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131500.052.245221
GTATCAA240150.040.944311
CAACGCA278500.033.725525
ATCAACG284800.033.0437053
TATCAAC304000.032.0123672
AACGCAG314100.030.5885096
GTGGTAT40550.030.2671641
GTACTAG61050.029.6549471
TCAACGC327250.029.016914
ACGCAGA331500.028.1046437
CGCAGAG334300.027.7702988
GTACATG336550.027.344081
TAGGCAT73950.026.6992765
TACATGG344000.026.4723342
ACATGGG338450.026.0140443
GCATCGT65100.025.908578
GGGCACC66300.025.66643794
GCAGAGT360300.025.3787929
CTGTGCG78700.025.016799
TAGTACT79200.024.9289284
Produced by FastQC (version 0.11.5)