FastQCFastQC Report
Thu 26 May 2016
ERR580271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7318352
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201350.27513024790280655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173020.23641934686934982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102840.14052344024993604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132100.049.7231371
GTATCAA237300.040.241771
CAACGCA276250.033.177675
ATCAACG282150.032.501893
TATCAAC304000.031.0884652
AACGCAG309100.030.2578436
GTACTAG60150.029.234851
GTGGTAT42700.028.9596181
TCAACGC326050.028.3702644
TAGGCAT71800.028.0831875
GGGCACC69550.027.772894
ACGCAGA333450.027.2871697
TAGTACT78800.026.9012724
CGCAGAG338950.026.8166648
CTAACGC28000.026.5234743
CTGTGCG79300.026.3718769
CTAGGCA80050.025.8940334
GTACATG342250.025.7174171
TCTAACG28900.025.5433962
TAACGCC29350.025.463114
Produced by FastQC (version 0.11.5)