##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580271_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7318352
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52945348898222	34.0	31.0	34.0	31.0	34.0
2	32.64643406056445	34.0	31.0	34.0	31.0	34.0
3	32.69649136854855	34.0	31.0	34.0	31.0	34.0
4	36.21655777147642	37.0	35.0	37.0	35.0	37.0
5	36.16235185189234	37.0	35.0	37.0	35.0	37.0
6	36.1989037969204	37.0	37.0	37.0	35.0	37.0
7	36.144063308242075	37.0	36.0	37.0	35.0	37.0
8	36.14109952623213	37.0	36.0	37.0	35.0	37.0
9	37.85340360780678	39.0	38.0	39.0	35.0	39.0
10-11	37.805668748920525	39.0	38.0	39.0	35.0	39.0
12-13	37.763067696115186	39.0	38.0	39.0	35.0	39.0
14-15	39.130687277682185	40.0	38.5	41.0	36.0	41.0
16-17	39.12139727632669	40.0	38.0	41.0	36.0	41.0
18-19	39.1184248174999	40.0	38.5	41.0	35.5	41.0
20-21	39.09108266451244	40.0	39.0	41.0	35.0	41.0
22-23	39.01277432405547	40.0	38.5	41.0	35.0	41.0
24-25	38.939423110558224	40.0	38.0	41.0	35.0	41.0
26-27	38.808483726937425	40.0	38.0	41.0	35.0	41.0
28-29	38.71133412276425	40.0	38.0	41.0	35.0	41.0
30-31	38.55969287894324	40.0	38.0	41.0	34.0	41.0
32-33	38.45194252749799	40.0	38.0	41.0	34.0	41.0
34-35	38.36261872891602	40.0	38.0	41.0	34.0	41.0
36-37	38.24555897283979	40.0	38.0	41.0	34.0	41.0
38-39	38.1272996980741	40.0	37.5	41.0	34.0	41.0
40-41	38.01526853313423	40.0	37.0	41.0	33.0	41.0
42-43	38.013900807176256	40.0	37.0	41.0	33.0	41.0
44-45	37.960961429567746	40.0	37.0	41.0	33.0	41.0
46-47	37.76281073935771	40.0	37.0	41.0	33.0	41.0
48-49	37.54240893304941	40.0	36.5	41.0	33.0	41.0
50-51	37.340613843116586	40.0	36.0	41.0	32.0	41.0
52-53	37.16489750697971	39.5	35.5	41.0	32.0	41.0
54-55	36.942530094207	39.0	35.0	41.0	31.5	41.0
56-57	36.69083346906517	39.0	35.0	41.0	31.0	41.0
58-59	36.43100714477795	38.5	35.0	40.5	31.0	41.0
60-61	36.17271326932621	38.0	35.0	40.0	31.0	41.0
62-63	35.86390016495517	37.0	35.0	40.0	30.0	41.0
64-65	35.56548106732226	37.0	35.0	40.0	30.0	41.0
66-67	35.254353712420496	36.0	34.0	39.5	29.5	41.0
68-69	34.950164189970636	36.0	34.0	39.0	29.0	41.0
70-71	34.65775464202869	35.0	34.0	39.0	29.0	40.5
72-73	34.365870895524026	35.0	34.0	38.0	29.0	40.0
74-75	34.03211856986382	35.0	34.0	37.0	29.0	39.5
76-77	32.51055831968728	34.0	31.5	35.5	26.0	38.5
78-79	33.41323722881873	35.0	33.0	36.0	28.0	39.0
80-81	33.40840191890196	35.0	33.0	36.0	29.0	38.5
82-83	33.191182113131475	35.0	33.0	36.0	28.0	37.0
84-85	32.9903685966458	35.0	33.0	35.0	27.5	37.0
86-87	32.79671420560257	35.0	33.0	35.0	27.0	36.5
88-89	32.62718239024305	35.0	33.0	35.0	27.0	36.0
90-91	32.464343133536076	35.0	33.0	35.0	27.0	36.0
92-93	32.3033241636915	35.0	33.0	35.0	26.0	36.0
94-95	32.17685327243073	35.0	33.0	35.0	26.0	35.0
96-97	32.060709364621985	35.0	32.5	35.0	25.0	35.0
98-99	31.96005494133105	35.0	32.0	35.0	25.0	35.0
100	30.072871870606935	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.030502394772973673
1101	2	-0.05169812342318636
1101	3	-0.02505459956670819
1101	4	-0.02507475783190216
1101	5	-0.04370441529670188
1101	6	-0.035137772853083504
1101	7	-0.04051646017980204
1101	8	-0.012267071149501874
1101	9	-0.05103339405784624
1101	10-11	-0.0663809491359757
1101	12-13	-0.05611952654393093
1101	14-15	-0.10770679376331316
1101	16-17	-0.03819310582876767
1101	18-19	-0.05092166093231043
1101	20-21	-0.03675654934000505
1101	22-23	-0.059671521116605675
1101	24-25	-0.11276621466232228
1101	26-27	-0.13960219654947537
1101	28-29	-0.09400542844821302
1101	30-31	-0.1272075066339653
1101	32-33	-0.13570376672290507
1101	34-35	-0.15388640850858337
1101	36-37	-0.16259250424635496
1101	38-39	-0.12198294448660363
1101	40-41	-0.0946324062907209
1101	42-43	-0.09210983380781101
1101	44-45	-0.0434400298244455
1101	46-47	-0.12438392491166894
1101	48-49	-0.11264948572575406
1101	50-51	-0.060600722700279164
1101	52-53	-0.022379076174168233
1101	54-55	-0.046128766365420404
1101	56-57	-0.10618491098993843
1101	58-59	-0.13155145248943967
1101	60-61	-0.12641522435976071
1101	62-63	-0.08672405646790082
1101	64-65	-0.04342716440844896
1101	66-67	-0.07584140702712006
1101	68-69	-0.11907612296931802
1101	70-71	-0.09264314282024344
1101	72-73	-0.08660232460034933
1101	74-75	-0.08236425775901068
1101	76-77	-0.14888490774365692
1101	78-79	-0.14904411162634545
1101	80-81	-0.06874246141223495
1101	82-83	-0.10612867763197897
1101	84-85	-0.15118190173251378
1101	86-87	-0.17484891538632752
1101	88-89	-0.1385466216122424
1101	90-91	-0.14590321346242519
1101	92-93	-0.13072286472353767
1101	94-95	-0.2059905147338057
1101	96-97	-0.26762237107973164
1101	98-99	-0.24419505717652967
1101	100	-0.34461026715082
1102	1	-0.049247593256389166
1102	2	-0.03363325987509569
1102	3	-0.03690176223928887
1102	4	-0.034388485671421165
1102	5	-0.019573055603920864
1102	6	-0.019429751519830063
1102	7	0.04777276795977059
1102	8	0.020827054745318208
1102	9	-0.017865781454901253
1102	10-11	0.009402477677028287
1102	12-13	-0.010871381316924555
1102	14-15	0.042280926789096895
1102	16-17	-0.04529160016729605
1102	18-19	-0.021480087808996018
1102	20-21	0.004118877693549905
1102	22-23	-0.03513291898492099
1102	24-25	-0.051620470097546445
1102	26-27	-0.06650311785777774
1102	28-29	-0.0600851599721679
1102	30-31	-0.047450497644881295
1102	32-33	-0.10995435018016764
1102	34-35	-0.05058788723644625
1102	36-37	-0.13902715843634184
1102	38-39	-0.16976874506708128
1102	40-41	-0.11285962494704904
1102	42-43	-0.04343313642136337
1102	44-45	-0.04261763282014641
1102	46-47	-0.011153299399460082
1102	48-49	0.01963921807622171
1102	50-51	-0.08120856312756786
1102	52-53	-0.08280120118266154
1102	54-55	-0.13501446953499396
1102	56-57	-0.0522376579528796
1102	58-59	-0.06450512896425664
1102	60-61	-0.09636719159937002
1102	62-63	-0.10983860536379098
1102	64-65	-0.07233341147378525
1102	66-67	-0.13962073473253866
1102	68-69	-0.15924745709978083
1102	70-71	-0.1303831333757799
1102	72-73	-0.11581725253998343
1102	74-75	-0.09506877975193362
1102	76-77	-0.10847307950963625
1102	78-79	0.0016911051860049042
1102	80-81	-0.08163853674275856
1102	82-83	-0.10302785805657066
1102	84-85	-0.11699404578666872
1102	86-87	-0.209470804900576
1102	88-89	-0.1444296924292061
1102	90-91	-0.13446016291950258
1102	92-93	-0.19796228515260594
1102	94-95	-0.15152159026359158
1102	96-97	-0.16773928705070063
1102	98-99	-0.1911426192290797
1102	100	-0.04769471412944881
1103	1	-0.020531707163257806
1103	2	-0.008401885185634228
1103	3	-0.002008539727889058
1103	4	-0.02849657204374978
1103	5	-0.05069228281603699
1103	6	0.00599278667163361
1103	7	-0.010897828690282552
1103	8	-0.022018581945147275
1103	9	-0.0601619610505395
1103	10-11	-0.010644323746682005
1103	12-13	-0.07197814114493184
1103	14-15	-0.02012460566050578
1103	16-17	-0.06341641595973613
1103	18-19	-0.04096749805025013
1103	20-21	-0.045091395660890043
1103	22-23	-0.07505843373726151
1103	24-25	-0.06232734436957088
1103	26-27	-0.06280604405262125
1103	28-29	-0.017053764173297736
1103	30-31	-0.06440310150879469
1103	32-33	-0.1236108258474431
1103	34-35	-0.15223116027216577
1103	36-37	-0.16332226098528935
1103	38-39	-0.09810992237267868
1103	40-41	-0.03210610336504516
1103	42-43	0.02284513865780724
1103	44-45	0.05072213830180772
1103	46-47	0.06697901839937259
1103	48-49	0.02322861012005717
1103	50-51	0.07846771307156075
1103	52-53	0.09049173102845742
1103	54-55	0.11011185040744209
1103	56-57	0.07007998218773537
1103	58-59	0.003137802355510644
1103	60-61	0.004669654327386752
1103	62-63	0.05827568904275182
1103	64-65	0.054068856847045765
1103	66-67	0.10205100102621856
1103	68-69	0.03357914029734843
1103	70-71	-0.017933738909441388
1103	72-73	0.021291379811472666
1103	74-75	-0.010498558789500123
1103	76-77	-0.06272555420124348
1103	78-79	4.632977711835906E-4
1103	80-81	-0.005962437732769388
1103	82-83	0.024927720452218693
1103	84-85	0.07511572492376217
1103	86-87	0.025847894376219926
1103	88-89	0.04906300949434694
1103	90-91	0.03267994528666662
1103	92-93	-0.03589827705605586
1103	94-95	-0.08493588953512443
1103	96-97	-0.06561912574277784
1103	98-99	-0.01211531302542923
1103	100	0.06453660941091499
1104	1	0.027569484491202445
1104	2	-0.020944345298858025
1104	3	0.0059747533557583665
1104	4	0.03760917244336781
1104	5	0.023111905790003107
1104	6	0.013866772181749809
1104	7	0.06486510729293116
1104	8	0.04434362558484395
1104	9	0.003272775083111412
1104	10-11	0.025366088383648844
1104	12-13	-0.0023121211124603747
1104	14-15	0.010942479338901023
1104	16-17	-0.002120877735734439
1104	18-19	-0.014557545162162455
1104	20-21	0.01198257797226887
1104	22-23	-0.0487346283609682
1104	24-25	0.02182355234051414
1104	26-27	-0.021450230283278415
1104	28-29	-0.023949821024316975
1104	30-31	-0.0405425567524631
1104	32-33	1.2027989703966568E-4
1104	34-35	0.007380106998908786
1104	36-37	-0.010656339927443526
1104	38-39	0.044779226049669774
1104	40-41	0.08102824478809367
1104	42-43	-0.02658065016993305
1104	44-45	-0.04064529508827519
1104	46-47	0.047270221534894574
1104	48-49	0.04562561712745605
1104	50-51	0.05529680369271972
1104	52-53	0.0021022460923987296
1104	54-55	0.05279552751262884
1104	56-57	0.059139941014294095
1104	58-59	0.017452066578158565
1104	60-61	-0.05457788120450857
1104	62-63	9.261502644903885E-4
1104	64-65	-0.008872075098004473
1104	66-67	-0.05131397779320679
1104	68-69	-0.07670012649865043
1104	70-71	-0.041110388816406385
1104	72-73	-0.007726989878541701
1104	74-75	-0.05249748986357616
1104	76-77	0.020775435544983623
1104	78-79	0.054432932356867525
1104	80-81	0.10200171365881516
1104	82-83	0.12694512019091775
1104	84-85	0.06900845622745777
1104	86-87	0.09611011774214973
1104	88-89	0.08571149122363408
1104	90-91	0.12113408044641005
1104	92-93	0.06995811076610181
1104	94-95	0.049424991734916546
1104	96-97	0.03471625966439973
1104	98-99	0.0011895881978993827
1104	100	0.1522318467520627
1105	1	-0.03971648698089325
1105	2	-9.831210916857458E-4
1105	3	-0.02291321229996157
1105	4	0.009959262458600904
1105	5	-0.026836854769477725
1105	6	-0.02848414874333116
1105	7	-0.05948387484250617
1105	8	-0.009332042605159074
1105	9	0.012411121572519335
1105	10-11	-0.011807156319306955
1105	12-13	0.011469072628010224
1105	14-15	-0.017223148690462153
1105	16-17	-0.039451718339527986
1105	18-19	-0.036281129153309166
1105	20-21	-0.022844454294727257
1105	22-23	-0.03538079925142057
1105	24-25	-0.043710307235720336
1105	26-27	-0.07720926642785741
1105	28-29	-0.0714155459931618
1105	30-31	-0.03722778040570063
1105	32-33	-0.11543933788443184
1105	34-35	-0.08829556119108872
1105	36-37	-0.04096294566072345
1105	38-39	-0.07210596990656626
1105	40-41	-0.08625634183426456
1105	42-43	-0.06508999790570869
1105	44-45	-0.03979361249583491
1105	46-47	-0.06588889770066686
1105	48-49	-0.04982432055430053
1105	50-51	-0.13806201087589187
1105	52-53	-0.14936984918526974
1105	54-55	-0.09735237444000688
1105	56-57	-0.17613661347954945
1105	58-59	-0.16770455992786282
1105	60-61	-0.13056818725630137
1105	62-63	-0.14809780763607705
1105	64-65	-0.09375644014439644
1105	66-67	-0.14842324243799254
1105	68-69	-0.13242149473019538
1105	70-71	-0.0645647580173474
1105	72-73	-0.08477310537500671
1105	74-75	-0.07545220527695307
1105	76-77	-0.132716601568994
1105	78-79	-0.03767148531380826
1105	80-81	-0.028018505147443307
1105	82-83	-0.015864739939253525
1105	84-85	0.03499571560981707
1105	86-87	0.016695355243889765
1105	88-89	-0.014022063685658281
1105	90-91	-0.013573300802725896
1105	92-93	-0.035264988527622165
1105	94-95	-0.015953210730110357
1105	96-97	-0.06008307560771797
1105	98-99	0.01343664539219347
1105	100	0.04811385755388997
1106	1	-0.002410408396144703
1106	2	-0.04455422972993972
1106	3	-0.02526643803764017
1106	4	0.010800002096658545
1106	5	0.00533120485981442
1106	6	3.5291330736697546E-4
1106	7	0.005892825041684091
1106	8	-0.014754936850557954
1106	9	0.007012698037165421
1106	10-11	0.022000120141612456
1106	12-13	0.034360997369169866
1106	14-15	-0.02404032626103003
1106	16-17	9.230948283871498E-4
1106	18-19	-0.0060052572734150544
1106	20-21	-0.0024389197257477235
1106	22-23	-0.015992544362092076
1106	24-25	-0.022142904485562553
1106	26-27	-0.06470053564829925
1106	28-29	0.031800437361482636
1106	30-31	0.019218600815889886
1106	32-33	-0.01084448567657148
1106	34-35	-0.028584094823884243
1106	36-37	-0.05407003907242114
1106	38-39	-0.010728995316483747
1106	40-41	-0.02413204836233973
1106	42-43	-0.006105502185342004
1106	44-45	0.04216030483656397
1106	46-47	0.06769904799359949
1106	48-49	0.004140415586540769
1106	50-51	-0.010225323527045305
1106	52-53	0.04261338017113303
1106	54-55	0.010449008673958815
1106	56-57	0.04109700199333588
1106	58-59	0.002152996767016191
1106	60-61	0.05507325099506488
1106	62-63	0.05136625173964404
1106	64-65	0.03107201961213235
1106	66-67	0.0034654077184015364
1106	68-69	0.08767204300243492
1106	70-71	0.04646012701559954
1106	72-73	0.05855366407240581
1106	74-75	0.00572406420952376
1106	76-77	-0.043252845954597774
1106	78-79	0.029960227292512798
1106	80-81	0.1082844346961167
1106	82-83	0.00579131017494916
1106	84-85	0.06004030814890626
1106	86-87	0.03251601833409268
1106	88-89	0.039924776951338004
1106	90-91	0.09496655249376573
1106	92-93	0.061081102403804266
1106	94-95	0.020286778825031604
1106	96-97	0.025844888810318878
1106	98-99	0.07746394693134206
1106	100	0.03927140326808143
1107	1	-0.018983782006543493
1107	2	0.011818498518849196
1107	3	-5.6057117596708395E-5
1107	4	0.0033555644230958137
1107	5	-0.0056505340763237655
1107	6	-0.0076813163937714535
1107	7	-0.030834242712579396
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2316	26-27	-0.14711424331886036
2316	28-29	-0.23967074339585537
2316	30-31	-0.18420591974233247
2316	32-33	-0.11198273934864034
2316	34-35	-0.1734109639730832
2316	36-37	-0.17168043062182647
2316	38-39	-0.21269393356255506
2316	40-41	-0.14196719189096285
2316	42-43	-0.1930325186180184
2316	44-45	-0.15158014750622328
2316	46-47	-0.18892929049745533
2316	48-49	-0.1823497158042926
2316	50-51	-0.15978595579469612
2316	52-53	-0.14758612198949805
2316	54-55	-0.21347132058902218
2316	56-57	-0.33061913470736926
2316	58-59	-0.30428237674435366
2316	60-61	-0.19273520960327062
2316	62-63	-0.22133048778570696
2316	64-65	-0.21589581910132694
2316	66-67	-0.2755525272378492
2316	68-69	-0.1815509930095942
2316	70-71	-0.19562258150729406
2316	72-73	-0.1394286362276702
2316	74-75	-0.16247225942236554
2316	76-77	-0.16496749304099723
2316	78-79	-0.25787372454465896
2316	80-81	-0.280180394482791
2316	82-83	-0.23877012511466233
2316	84-85	-0.1466487963326415
2316	86-87	-0.21252301812173613
2316	88-89	-0.14766605099683972
2316	90-91	-0.22579768642119546
2316	92-93	-0.20639837430667995
2316	94-95	-0.20005660980439188
2316	96-97	-0.20379408689663592
2316	98-99	-0.1443892428465503
2316	100	-0.3221019560587344
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	3.0
13	65.0
14	246.0
15	889.0
16	2363.0
17	5420.0
18	10589.0
19	16475.0
20	24229.0
21	36874.0
22	48004.0
23	49248.0
24	45675.0
25	44290.0
26	50251.0
27	61634.0
28	75978.0
29	94155.0
30	116895.0
31	146845.0
32	188050.0
33	251398.0
34	386757.0
35	576153.0
36	1009556.0
37	1816026.0
38	1781742.0
39	478347.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80845211152474	21.208237462185615	13.404146115691457	23.579164310598195
2	15.287494564955159	20.78322874673641	40.563962532806755	23.365314155501675
3	17.852204131694766	27.695874536228303	30.058213322955634	24.393708009121298
4	12.342638417863707	17.392242442428575	39.31064459747724	30.954474542230482
5	12.649979242525738	39.03011897216749	34.9744583310444	13.345443454262359
6	29.525638846362597	38.610107089742115	18.07547277063075	13.788781293264545
7	26.18000762201059	32.937115406504724	23.673653327510472	17.20922364397422
8	26.014300468911294	35.49729823244739	20.098261745808372	18.39013955283294
9	26.725807472041435	14.98606017484256	21.404323798574	36.883808554542
10-11	25.37597181435202	26.207943897617692	27.825764181727415	20.590320106302876
12-13	25.6368377125548	24.097883839358282	28.187884752132586	22.077393695954335
14-15	23.19400176530394	25.21824610173433	25.849206641472698	25.738545491489024
16-17	21.84147445854409	27.867199528308817	27.031054483269617	23.26027152987748
18-19	21.447632306001914	27.731143420155636	29.251090522487456	21.570133751354998
20-21	22.82581099697773	26.871073732861205	28.6965018560225	21.606613414138565
22-23	23.224335972408593	26.42203295336844	28.36083091243499	21.992800161787976
24-25	22.618827605115673	26.62850367631812	28.86395323915475	21.888715479411463
26-27	22.392626426104385	26.884626358328045	28.325522656701356	22.39722455886621
28-29	21.762032702099347	27.281196036668163	28.747553662290105	22.20921759894238
30-31	22.71726594718388	26.83198919955946	28.572972662751322	21.87777219050534
32-33	22.891454644099905	27.102980483044632	27.936713901926925	22.068850970928544
34-35	22.741970317011486	27.073686995182417	28.61745409572265	21.566888592083448
36-37	22.5793029205743	26.75231520103656	28.355570823745737	22.312811054643404
38-39	22.62380088009703	26.81041074656299	28.73259632995932	21.833192043380663
40-41	22.31552696576627	26.08862303269644	29.255692241470605	22.340157760066685
42-43	22.20227009260568	26.417465311728645	28.50716203032452	22.873102565341156
44-45	22.225999782182978	27.092987234747305	28.421028826046875	22.259984157022846
46-47	22.276948951371644	26.76970596100478	27.907591492364425	23.04575359525915
48-49	22.866211504005985	26.703228360584923	28.005028098397894	22.425532037011198
50-51	22.579377161154483	26.678410100893053	27.776082531367148	22.966130206585326
52-53	22.129582175541522	27.110492574497407	27.65667400861944	23.103251241341628
54-55	22.009331484563926	27.070966317259813	27.612745303682512	23.306956894493744
56-57	22.79712092349521	26.67709103326603	27.283176442880986	23.242611600357773
58-59	22.002934290303976	27.036903145121105	27.965045145476807	22.99511741909811
60-61	22.344308378644186	27.4282661838975	27.344784615777755	22.882640821680557
62-63	22.448729443728908	26.860617835457184	27.244514586990213	23.446138133823695
64-65	21.735218469478703	26.85222415160162	27.53727644127545	23.875280937644227
66-67	22.320429456613308	26.653906042359445	27.356815341718494	23.668849159308753
68-69	21.814608293035473	26.74194506227135	27.597802906255943	23.84564373843724
70-71	21.873824621844655	26.338644052672795	27.69860333731728	24.088927988165274
72-73	22.22799197333482	26.731228815096657	27.530841160702956	23.509938050865564
74-75	22.386735691309365	27.012295953973975	27.245099021245945	23.355869333470718
76-77	22.074244813831122	26.847581523293133	27.005663328321294	24.072510334554448
78-79	21.98852265594484	26.821959874730744	27.152302632500415	24.037214836824
80-81	21.724419610110925	27.291381304432903	27.281727270044044	23.702471815412128
82-83	22.38469129850637	26.570762152760697	27.98417274209047	23.060373806642463
84-85	22.523077045006815	26.86065827908846	26.952525093933577	23.663739581971146
86-87	21.47291889843288	27.486022160255636	27.3443255740577	23.69673336725379
88-89	22.115311695127886	26.417855953571866	27.32884359677582	24.137988754524432
90-91	22.090002371471666	26.388870384896478	27.358741489843087	24.16238575378877
92-93	22.31609059152035	26.554255094560315	27.15124799391119	23.978406320008144
94-95	21.412337895256815	26.483882274088455	27.578983040193712	24.524796790461025
96-97	21.67409618530742	26.453231268406547	27.28893340488992	24.583739141396112
98-99	21.186651898116683	26.924051829849034	27.14005963717575	24.749236634858534
100	21.425094843809013	26.694371461974754	26.64429185384461	25.236241840371626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	699.0
1	502.0
2	404.5
3	523.5
4	471.5
5	496.0
6	759.5
7	1445.0
8	2394.5
9	3064.5
10	3026.0
11	2863.0
12	3250.0
13	3791.5
14	4447.0
15	5167.5
16	5867.0
17	6721.0
18	7788.0
19	8845.5
20	9772.0
21	10810.0
22	12104.0
23	13799.0
24	15874.0
25	18322.5
26	22094.0
27	26916.0
28	33261.0
29	44664.0
30	60721.5
31	76278.0
32	97815.5
33	122498.0
34	144856.5
35	167936.0
36	191957.0
37	226358.0
38	241707.0
39	234838.5
40	224302.5
41	211372.5
42	210061.0
43	219363.5
44	234521.5
45	269092.5
46	300491.0
47	317178.0
48	330276.0
49	326778.5
50	317265.0
51	318008.0
52	314922.5
53	300712.5
54	287449.0
55	255714.0
56	224767.5
57	188570.5
58	148141.5
59	131808.0
60	106049.5
61	74349.5
62	54606.0
63	42898.0
64	30096.5
65	15658.0
66	9086.0
67	6281.0
68	4467.0
69	3031.0
70	2149.5
71	1541.5
72	1027.0
73	639.5
74	391.5
75	245.0
76	125.5
77	63.0
78	37.5
79	16.5
80	12.5
81	8.0
82	4.0
83	3.5
84	4.0
85	2.5
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005055783050610301
2	0.032862589828966955
3	0.0013117707374556457
4	0.0012981064589404827
5	0.006914124928672467
6	5.465711406065191E-5
7	9.974923316068973E-4
8	0.0075153531833396375
9	0.0015167349151830904
10-11	2.3912487401535207E-4
12-13	5.465711406065191E-5
14-15	8.95010242743175E-4
16-17	6.14892533182334E-5
18-19	9.701637745765713E-4
20-21	1.9129989921228167E-4
22-23	0.001803684764001513
24-25	0.0012639457626525752
26-27	0.0022409416764867284
28-29	1.9813203846986318E-4
30-31	0.001803684764001513
32-33	0.0017148669536529537
34-35	7.173746220460563E-4
36-37	0.004167604947124708
38-39	9.291709390310825E-4
40-41	0.0037850051487001444
42-43	0.0038328301235032148
44-45	0.0038669908197911224
46-47	0.002363920183123195
48-49	0.00922338799773501
50-51	0.00688679637164214
52-53	0.004953300961746579
54-55	0.004522876188518946
56-57	0.003299923261411859
58-59	0.0054452149882924464
60-61	0.007774974475127734
62-63	0.0032589304258663697
64-65	0.0030676305266540884
66-67	0.0036483623635485147
68-69	0.0018993347136076536
70-71	0.0031222876407147404
72-73	0.006743321447232929
74-75	0.007911617260279363
76-77	0.004174437086382289
78-79	0.0026713664497143618
80-81	0.0022955987905473804
82-83	0.004700511809216064
84-85	0.0020359774987592835
86-87	0.003422901768048326
88-89	4.2359263397005227E-4
90-91	0.0015235670544406718
92-93	9.564994960614084E-5
94-95	0.002281934512032217
96-97	0.0018378454602894204
98-99	0.0013390992944859717
100	5.465711406065191E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7318352.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.26929744362607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.19766569760044	38.797756652889056
2	16.91821919034668	18.701161772954666
3	5.997958921243726	9.945089270186102
4	2.5887597201086567	5.723157239230542
5	1.37588772616745	3.8022173993291575
6	0.8201249826418098	2.7196638963947315
7	0.5117657605212459	1.9799453827798574
8	0.326029742788263	1.441554786370673
9	0.24032921750254524	1.195454430588787
>10	0.9480531895767447	8.673812772537918
>50	0.04425593412656244	1.6859510454609385
>100	0.028001017414514304	3.032511213699919
>500	0.002054294629377441	0.7903428822817138
>1k	7.952047395560572E-4	0.6374823448523813
>5k	0.0	0.0
>10k+	9.94005924112594E-5	0.8738989104435988
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20135	0.27513024790280655	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17302	0.23641934686934982	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10284	0.14052344024993604	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3664278515162976E-5
2	0.0	0.0	0.0	0.0	1.3664278515162976E-5
3	0.0	0.0	0.0	0.0	1.3664278515162976E-5
4	0.0	0.0	0.0	1.3664278515162976E-5	2.732855703032595E-5
5	0.0	0.0	0.0	1.3664278515162976E-5	2.732855703032595E-5
6	1.3664278515162976E-5	0.0	0.0	1.3664278515162976E-5	4.099283554548893E-5
7	1.3664278515162976E-5	0.0	0.0	2.732855703032595E-5	4.099283554548893E-5
8	2.732855703032595E-5	0.0	0.0	2.732855703032595E-5	4.099283554548893E-5
9	2.732855703032595E-5	0.0	0.0	4.099283554548893E-5	4.099283554548893E-5
10-11	1.5030706366679275E-4	0.0	0.0	4.099283554548893E-5	4.782497480307041E-5
12-13	1.5030706366679275E-4	0.0	0.0	5.46571140606519E-5	1.3664278515162977E-4
14-15	1.7080348143953721E-4	6.832139257581488E-6	0.0	5.46571140606519E-5	1.776356206971187E-4
16-17	1.9129989921228167E-4	1.3664278515162976E-5	0.0	7.515353183339636E-5	1.9129989921228167E-4
18-19	1.9129989921228167E-4	1.3664278515162976E-5	0.0	8.198567109097785E-5	1.9129989921228167E-4
20-21	1.9129989921228167E-4	1.3664278515162976E-5	0.0	1.093142281213038E-4	1.9813203846986315E-4
22-23	2.0496417772744466E-4	1.3664278515162976E-5	0.0	1.844677599547002E-4	2.2546059550018912E-4
24-25	2.3912487401535207E-4	3.416069628790744E-5	0.0	4.372569124852152E-4	2.459570132729336E-4
26-27	2.5962129178809653E-4	5.46571140606519E-5	0.0	0.0013869242692890421	2.5962129178809653E-4
28-29	2.5962129178809653E-4	6.14892533182334E-5	0.0	0.0047756653410494605	2.5962129178809653E-4
30-31	3.0744626659116696E-4	6.832139257581489E-5	0.0	0.018098336893333362	2.8011770956084105E-4
32-33	3.1427840584874846E-4	6.832139257581489E-5	0.0	0.05279877218258974	3.1427840584874846E-4
34-35	3.894319376821448E-4	7.515353183339636E-5	0.0	0.10976514931230419	3.4160696287907443E-4
36-37	4.645854695155412E-4	9.564994960614082E-5	0.0	0.20109035476839593	3.4160696287907443E-4
38-39	4.645854695155412E-4	1.229785066364668E-4	0.0	0.33549903038279655	3.4160696287907443E-4
40-41	5.055783050610302E-4	1.4347492440921128E-4	0.0	0.4792540725015686	3.4160696287907443E-4
42-43	5.055783050610302E-4	1.5030706366679275E-4	0.0	0.6232414073550985	3.4160696287907443E-4
44-45	5.055783050610302E-4	1.5030706366679275E-4	0.0	0.7990596790097005	3.621033806518189E-4
46-47	5.055783050610302E-4	1.5030706366679275E-4	0.0	1.0101522856511957	4.030962161973078E-4
48-49	5.055783050610302E-4	1.6397134218195574E-4	0.0	1.243285373537649	4.4408905174279673E-4
50-51	5.260747228337746E-4	1.776356206971187E-4	0.0	1.48588780643511	4.645854695155412E-4
52-53	5.329068620913561E-4	1.776356206971187E-4	0.0	1.7204966364012009	5.055783050610301E-4
54-55	5.329068620913561E-4	1.9129989921228167E-4	0.0	1.978751500337781	5.329068620913561E-4
56-57	5.329068620913561E-4	2.0496417772744466E-4	0.0	2.275307336952363	5.397390013489376E-4
58-59	5.329068620913561E-4	2.1179631698502613E-4	0.0	2.6188068024057873	5.670675583792635E-4
60-61	5.329068620913561E-4	2.459570132729336E-4	0.0	2.974194190167404	5.943961154095895E-4
62-63	5.329068620913561E-4	2.459570132729336E-4	0.0	3.3390714193578006	6.01228254667171E-4
64-65	5.329068620913561E-4	2.459570132729336E-4	0.0	3.7066541756942	6.148925331823339E-4
66-67	5.329068620913561E-4	2.5962129178809653E-4	0.0	4.110358452285433	6.148925331823339E-4
68-69	5.465711406065191E-4	2.5962129178809653E-4	0.0	4.562885196011342	6.148925331823339E-4
70-71	5.670675583792635E-4	2.8011770956084105E-4	0.0	5.055400450811877	6.148925331823339E-4
72-73	5.73899697636845E-4	3.006141273335855E-4	0.0	5.551598228672248	6.763817865005674E-4
74-75	5.73899697636845E-4	3.006141273335855E-4	0.0	6.0346714670188035	6.900460650157304E-4
76-77	5.73899697636845E-4	3.006141273335855E-4	0.0	6.569354685317132	7.242067613036378E-4
78-79	6.285568116974969E-4	3.006141273335855E-4	0.0	7.138909142386154	7.378710398188008E-4
80-81	6.285568116974969E-4	3.006141273335855E-4	0.0	7.743396327479192	7.378710398188008E-4
82-83	6.353889509550784E-4	3.006141273335855E-4	0.0	8.361670769594028	7.515353183339637E-4
84-85	6.422210902126599E-4	3.006141273335855E-4	0.0	8.961238814421607	7.515353183339637E-4
86-87	6.422210902126599E-4	3.006141273335855E-4	0.0	9.57898718181361	7.651995968491267E-4
88	6.558853687278228E-4	3.006141273335855E-4	0.0	10.064260369001108	7.788638753642897E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13210	0.0	49.723137	1
GTATCAA	23730	0.0	40.24177	1
CAACGCA	27625	0.0	33.17767	5
ATCAACG	28215	0.0	32.50189	3
TATCAAC	30400	0.0	31.088465	2
AACGCAG	30910	0.0	30.257843	6
GTACTAG	6015	0.0	29.23485	1
GTGGTAT	4270	0.0	28.959618	1
TCAACGC	32605	0.0	28.370264	4
TAGGCAT	7180	0.0	28.083187	5
GGGCACC	6955	0.0	27.7728	94
ACGCAGA	33345	0.0	27.287169	7
TAGTACT	7880	0.0	26.901272	4
CGCAGAG	33895	0.0	26.816664	8
CTAACGC	2800	0.0	26.523474	3
CTGTGCG	7930	0.0	26.371876	9
CTAGGCA	8005	0.0	25.894033	4
GTACATG	34225	0.0	25.717417	1
TCTAACG	2890	0.0	25.543396	2
TAACGCC	2935	0.0	25.46311	4
>>END_MODULE