FastQCFastQC Report
Thu 26 May 2016
ERR580270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677706
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92080.25037346650330394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68950.1874810003844788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38900.10577245706970596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65050.048.8685341
GTATCAA122750.043.289931
CAACGCA145000.035.510145
GTGGTAT20600.035.1547051
ATCAACG147600.034.951673
TATCAAC152900.034.5710332
AACGCAG161150.032.6662256
TCAACGC165600.031.3496554
ACGCAGA177850.028.858137
CGCAGAG183100.028.0563558
GTACTAG24050.025.4189831
TATTCTC20500.025.2316175
GCAGAGT200300.025.2268149
TACCCCG3600.024.8174635
GTACATG193150.024.3464261
GGGCTAT37300.024.3197846
TACATGG197900.023.4781952
AGGGCTA37900.023.3251765
GGCTATT39550.022.8167697
GCTATTG38050.022.7280718
Produced by FastQC (version 0.11.5)