FastQCFastQC Report
Thu 26 May 2016
ERR580270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677706
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76510.20803729281242164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61930.16839301455853187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38000.10332527939971275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66950.050.691211
GTATCAA121200.042.545151
CAACGCA147350.033.87365
ATCAACG150800.033.223763
TATCAAC156150.032.8985672
AACGCAG164500.031.0559446
TCAACGC166500.030.2599034
GTGGTAT22150.029.2854161
ACGCAGA180900.027.5909757
CGCAGAG185950.026.7405648
GTACTAG25350.026.1449131
AGGGCTA36200.025.5767085
GGGCTAT36800.025.5424926
TACCCCG3550.025.1542995
CTATTGA37050.025.116449
GCTATTG36600.025.0400168
GGCTATT37550.024.7827
GCAGAGT202200.024.4055869
ATTTAGG38750.024.1394541
GAGTACT79750.022.39396912-13
Produced by FastQC (version 0.11.5)