FastQCFastQC Report
Thu 26 May 2016
ERR580268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4319199
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51850.12004540656728249No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54700.047.1903081
GTATCAA94900.041.469331
CAACGCA112700.033.1627585
ATCAACG114350.032.770263
TATCAAC121000.031.9036712
AACGCAG126550.030.2622836
TCAACGC125900.029.8752754
GTACATG177750.026.2403621
ACGCAGA141250.026.180777
TACATGG180350.025.2111842
CGCAGAG147100.025.1070658
GTACTAG20900.024.5215551
ACATGGG182650.024.0685353
TGATCGC15250.022.8043889
CATGGGG147000.021.9089164
GTGATCG15900.021.8685868
GCAGAGT164050.021.8577169
GTATAAG26250.021.8523581
GTGGTAT25500.019.9136731
CGTTAAC6950.019.6191861
Produced by FastQC (version 0.11.5)