FastQCFastQC Report
Thu 26 May 2016
ERR580268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4319199
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44860.10386185031067102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58300.044.2510531
GTATCAA101550.040.220071
CAACGCA117050.033.1242525
ATCAACG122000.031.7421233
TATCAAC128900.031.1725082
AACGCAG131900.029.8574526
TCAACGC133350.029.216634
ACGCAGA148650.025.955647
GTACATG186600.024.6685161
CGCAGAG157000.024.4859648
TACATGG187300.024.1627882
ACATGGG189750.022.9844863
GTACTAG21650.022.6558461
CATGGGG148950.021.3607644
GTGGTAT27450.021.305131
GCAGAGT175850.021.2465469
ATGGGGG99550.019.308375
GTATAAG24500.018.6728421
TAGTACT29050.018.6045994
AGAGTAC154900.018.49144210-11
Produced by FastQC (version 0.11.5)