FastQCFastQC Report
Thu 26 May 2016
ERR580267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164109
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66070.1586653951661688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.12790251167776828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54200.046.1547281
GTATCAA93950.040.2404751
CAACGCA110100.033.2205925
ATCAACG111900.032.6493033
TATCAAC118400.031.533912
TCAACGC121250.030.3233134
AACGCAG125450.029.966516
ACGCAGA141500.025.8362567
GTACATG165200.025.1905161
GTACTAG18050.024.4880661
TACATGG164900.024.4667152
CGCAGAG149500.024.3279868
GTGGTAT26300.023.6005461
ACATGGG172100.022.6493973
GCAGAGT168550.020.9401689
CATGGGG136650.020.5751574
GTATAAG22900.020.1230771
TAGTACT24300.020.122354
TACCCCG4700.020.00545
TGGTATC30600.018.9013462
Produced by FastQC (version 0.11.5)