FastQCFastQC Report
Thu 26 May 2016
ERR580266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9062583
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226700.25014943311415744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182900.20181884127295716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109510.12083751398469951No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139300.044.5009571
GTATCAA245700.040.1802251
CAACGCA271450.034.4054535
GTACTAG79350.033.7857061
ATCAACG280950.033.2148363
TATCAAC303950.032.1101722
GTGGTAT42050.031.2063711
AACGCAG310400.030.8156936
GGGCACC88500.030.16398694
GCATCGT81250.029.2709758
TCAACGC330250.028.4114344
TCTAACG36000.028.0907042
CTGTGCG107200.028.0158739
ACGCAGA333950.027.9521397
TAGGCAT100950.027.8568975
GTACATG354750.027.8553541
CTAACGC36000.027.5676293
TAGTACT109450.027.329674
CGCAGAG343150.027.147498
TAACGCC37400.027.0370884
Produced by FastQC (version 0.11.5)