FastQCFastQC Report
Thu 26 May 2016
ERR580266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9062583
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195770.21602009051944682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166470.18368935214165763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106880.11793547159788771No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137650.044.157991
GTATCAA239300.039.7804151
CAACGCA271350.033.3435025
GGGCACC90400.032.29063894
ATCAACG280650.032.1885223
GTACTAG77100.032.010461
TATCAAC309650.030.6928442
AACGCAG315450.029.7532166
GTGGTAT42400.029.6027741
CTGTGCG112300.028.542439
TAGTACT113300.027.9187184
TCAACGC328950.027.7192924
GTACATG342300.027.1647911
ACGCAGA337100.026.8244157
TACATGG345750.026.5773472
CGCAGAG347850.025.9684098
ACATGGG342250.025.694673
CTAGTAC130200.025.5584773
GTACTGT131300.025.1996356
AGGGCTA83400.025.0786575
Produced by FastQC (version 0.11.5)