FastQCFastQC Report
Thu 26 May 2016
ERR580265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11413016
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331390.29036146098454607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252420.22116853248957155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159710.13993671786668835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187800.047.455431
GTATCAA333100.040.3232771
CAACGCA366700.034.817295
GTACTAG94300.033.6140251
ATCAACG379900.033.610633
TATCAAC411150.032.383182
AACGCAG419250.031.268146
TCAACGC437850.029.4838074
GTGGTAT58950.028.8002131
GCATCGT100550.028.4665748
GGGCACC112650.028.28713494
ACGCAGA452150.028.1608317
TAGTACT128800.028.1152174
CGCAGAG460250.027.6027418
TAGGCAT119700.027.5790375
GTACATG458050.027.526831
CTGTGCG127050.027.4496529
TACATGG463600.026.761552
CTAGGCA130150.026.4504764
ACATGGG455800.026.1771433
Produced by FastQC (version 0.11.5)