FastQCFastQC Report
Thu 26 May 2016
ERR580265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11413016
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289810.25392937326995774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230070.201585628198541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153420.1344254665024565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177550.047.1811071
GTATCAA320550.040.3583831
CAACGCA365350.033.7824065
ATCAACG370900.033.328033
GTACTAG88650.032.1877821
TATCAAC405550.031.7912312
AACGCAG420650.030.0881676
GGGCACC107950.029.69711794
TCAACGC430800.028.9556944
GTGGTAT54350.028.283251
ACGCAGA450200.027.4136947
GTACATG449450.027.3822651
TAGTACT132750.027.0500224
CTGTGCG129050.026.9131669
CGCAGAG462750.026.649918
TACATGG459450.026.5066622
ACATGGG452150.025.8733733
CTAGTAC149600.024.7888343
CTAACGC41400.024.5224633
TCTAACG42500.024.2203882
Produced by FastQC (version 0.11.5)