FastQCFastQC Report
Thu 26 May 2016
ERR580264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10201539
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208930.20480243226046582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159360.15621172452509371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT27850.044.411381
GGGCACC128650.041.71967794
GTATCAA305900.037.65071
GGTATCA180750.036.7904241
CAACGCA295750.035.7586825
TATTCTC43550.035.63225
GTACTAG146700.034.9109341
ATCAACG316650.033.5507773
TACCCCG7100.033.1152845
TAGGCAT172150.032.4235465
TATCAAC369300.031.200472
GCATCGT155150.030.961058
AACGCAG381600.029.316266
CTAGGCA199600.029.2154184
AGGGCTA108650.029.213995
ACGCAGA362150.029.1897457
CGCAGAG364150.029.0285748
CTGTGCG201650.028.9730199
TAGTACT204100.028.7556064
ACTGTGC208000.028.381998
Produced by FastQC (version 0.11.5)