FastQCFastQC Report
Thu 26 May 2016
ERR580264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10201539
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183570.1799434379459805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144000.1411551727636389No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC126890.12438319355540374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC128300.043.2698494
GTGGTAT29300.037.864671
GGTATCA179300.037.5450331
GTATCAA300000.036.8557971
TACCCCG6750.035.5118375
GTACTAG142450.034.816071
CAACGCA304000.033.5964135
TATTCTC44000.032.793855
TAGGCAT170450.032.3450475
ATCAACG322950.031.6837273
TATCAAC373550.029.6068842
CTAGGCA194550.029.5702344
AGGGCTA106350.029.3894125
CTGTGCG204750.029.358389
ACTGTGC211400.028.8572658
TAGTACT209650.028.4717044
AACGCAG387300.027.8982496
GTACTGT220350.027.3652296
CAGTACG18050.027.3399379
GAACCGT17050.027.2895436
Produced by FastQC (version 0.11.5)