FastQCFastQC Report
Thu 26 May 2016
ERR580263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847526
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53520.11040683433157449No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58150.042.6898041
GTATCAA104250.040.904511
CAACGCA117700.034.8278125
ATCAACG124300.033.0943953
TATCAAC129000.032.7288782
AACGCAG133800.031.5010686
TCAACGC131700.031.3416484
ACGCAGA153250.026.5210767
GTACTAG23050.025.7003821
CGCAGAG162350.024.8893018
GTACATG199850.024.4663471
TACATGG203400.023.623512
ACATGGG209350.022.1871513
GTGGTAT29700.021.528931
GCAGAGT183450.021.2389649
TAGTACT32450.020.8622364
CATGGGG162900.020.3749584
GCATCGT25750.020.07168
CTAGTAC33650.019.4199143
CTGTGCG37900.018.4775169
Produced by FastQC (version 0.11.5)