FastQCFastQC Report
Thu 26 May 2016
ERR580263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847526
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49280.10166010455642735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64000.045.7749521
GTATCAA106300.039.586571
CAACGCA123950.032.7611125
ATCAACG128100.031.5903473
TATCAAC133500.031.190442
AACGCAG137750.029.9548726
TCAACGC137400.029.6570744
ACGCAGA157450.025.6099747
CGCAGAG165850.024.0861848
GTGGTAT27250.023.5444561
GTACATG195400.022.9600181
TACATGG199150.022.2300072
GTACTAG22250.021.6265091
GCAGAGT184500.021.2948449
ACATGGG204450.021.0575943
GAGTACT64350.019.35280612-13
CATGGGG164700.018.5487944
CGTTAAC6400.018.427921
CAGAGTA177250.018.39970610-11
GTATAAG26050.018.1095871
Produced by FastQC (version 0.11.5)