FastQCFastQC Report
Thu 26 May 2016
ERR580262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5046770
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156830.31075321443220116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129160.25592606756400627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77090.1527511655970056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83050.053.7943081
GTATCAA150000.042.7010151
CAACGCA178700.034.8326535
ATCAACG179100.034.8056343
TATCAAC189950.033.4415632
AACGCAG195900.032.2610476
TCAACGC193800.032.238994
GTGGTAT29150.031.1365951
ACGCAGA214700.028.9555157
GTACTAG23150.028.6431311
CGCAGAG218150.028.3465278
GTACATG190700.026.6333271
TACATGG190900.026.0831812
ACATGGG190500.025.1466873
GCAGAGT243000.024.9087459
TGGTATC36000.024.1623842
CATGGGG147050.022.347164
GAGTACT141100.022.2145112-13
CAGAGTA238200.021.5742910-11
GCATCGT25450.021.0482648
Produced by FastQC (version 0.11.5)