FastQCFastQC Report
Thu 26 May 2016
ERR580262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5046770
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134190.26589283838970273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118620.23504142253362054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73280.14520178252624946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78550.052.732071
GTATCAA140800.040.6714631
CAACGCA169700.033.0139545
ATCAACG171000.032.7076723
TATCAAC179150.031.6495132
AACGCAG184100.030.9659446
TCAACGC182150.030.8867654
GTGGTAT28450.028.0938851
ACGCAGA206750.027.0507147
CGCAGAG211000.026.4613088
GTACATG188250.026.0991541
TACATGG186050.025.9523892
ACATGGG187550.024.8595583
GTACTAG24800.024.6454541
GCAGAGT233950.023.5237679
CATGGGG144050.022.4156284
TGGTATC34150.021.6145152
CTGTGCG31000.021.0729879
ATGGGGG101550.020.9199985
GAGTACT137350.020.56412512-13
Produced by FastQC (version 0.11.5)