FastQCFastQC Report
Thu 26 May 2016
ERR580261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9094176
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181200.19924839809566033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133300.14657732597213866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110700.045.7898521
GTATCAA197500.041.16481
CAACGCA228800.034.2460335
ATCAACG234500.033.3133933
GTACATG347200.033.0722731
TACATGG353300.032.0957872
TATCAAC251450.032.0272872
AACGCAG256300.031.3451446
GTACTAG47600.031.1172831
ACATGGG354950.031.0002333
TCAACGC258600.030.5002754
CATGGGG289600.029.3941024
ATGGGGG214050.028.4590575
TAACGCC22150.027.7995554
CTAACGC22150.027.7989433
ACGCAGA287400.027.2845157
TCTAACG23350.026.1740472
CGCAGAG298800.026.0861558
TGGGGGG201850.025.7656826
TAGTACT67550.024.7722194
Produced by FastQC (version 0.11.5)