FastQCFastQC Report
Thu 26 May 2016
ERR580261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9094176
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156310.1718792334786571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124030.13638398904969512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112250.045.567541
GTATCAA195150.040.351461
CAACGCA231050.032.8525965
ATCAACG235200.032.3344763
GTACATG336350.031.8122121
TACATGG341450.030.9518762
TATCAAC255350.030.746692
AACGCAG257000.030.008236
GTACTAG48050.029.6457371
ACATGGG345550.029.6123033
TCAACGC259250.029.4791164
CATGGGG279150.027.6301774
ATGGGGG213400.026.4515865
CTAACGC20250.026.2287773
ACGCAGA290100.026.017337
TGGGGGG184200.025.8454886
TCTAACG20550.025.6226482
TAACGCC20550.025.387734
CGCAGAG300900.025.0052788
TAGTACT65050.025.0000864
Produced by FastQC (version 0.11.5)