FastQCFastQC Report
Thu 26 May 2016
ERR580260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4344240
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55130.12690367014713738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43710.10061598806695762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.046.21031
GTATCAA93750.041.8772431
CAACGCA109950.034.9732445
ATCAACG114500.033.6284073
TATCAAC120450.032.4382252
AACGCAG124850.031.5401136
TCAACGC125400.030.8553264
ACGCAGA139050.027.237797
GTACTAG23450.026.4663451
CGCAGAG142850.026.3152528
GCATCGT22200.023.706258
GTACATG167000.023.3400041
GCAGAGT162400.022.6614959
TACATGG171000.022.2991162
TAGGCAT32950.021.3999945
ACATGGG172050.021.3687923
CTAGGCA34800.021.0752724
TGATCGC14400.020.8896089
GTATAAG27100.020.6462211
GTGATCG14450.020.4866288
Produced by FastQC (version 0.11.5)