FastQCFastQC Report
Thu 26 May 2016
ERR580260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4344240
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48110.11074434193322652No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57600.045.676371
GTATCAA97850.039.8014641
CAACGCA111700.033.6187445
ATCAACG114300.032.731033
TATCAAC123450.030.9885432
AACGCAG127100.030.3951426
TCAACGC124950.030.3546434
ACGCAGA141050.026.4560667
GTACTAG17850.026.1503091
CGCAGAG145650.025.2978388
GTACATG167150.023.0742071
TACATGG169850.022.0526432
CGTTAAC8050.021.6713331
GCAGAGT165500.021.6386349
ACATGGG171850.021.113523
GTGGTAT26650.020.8768331
CATGGGG131750.019.727064
TAGGCAT29600.019.2123975
AGAGTAC151700.018.37022410-11
GAGTACT65800.018.2481112-13
Produced by FastQC (version 0.11.5)