##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580260_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4344240
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5484045540762	34.0	31.0	34.0	31.0	34.0
2	32.741429801300114	34.0	31.0	34.0	31.0	34.0
3	32.82441140452645	34.0	31.0	34.0	31.0	34.0
4	36.28952359906451	37.0	37.0	37.0	35.0	37.0
5	36.22396184372871	37.0	37.0	37.0	35.0	37.0
6	36.24940012522328	37.0	37.0	37.0	35.0	37.0
7	36.217663849142774	37.0	37.0	37.0	35.0	37.0
8	36.206828812404474	37.0	37.0	37.0	35.0	37.0
9	37.99511583153785	39.0	38.0	39.0	35.0	39.0
10-11	37.94676479660424	39.0	38.0	39.0	35.0	39.0
12-13	37.916294679851944	39.0	38.0	39.0	35.0	39.0
14-15	39.329027171611145	41.0	39.0	41.0	36.0	41.0
16-17	39.31319344695505	41.0	39.0	41.0	36.0	41.0
18-19	39.30454820636061	41.0	39.0	41.0	36.0	41.0
20-21	39.286814034215425	41.0	39.0	41.0	36.0	41.0
22-23	39.22194998434709	41.0	39.0	41.0	36.0	41.0
24-25	39.146904291659766	40.5	39.0	41.0	35.5	41.0
26-27	39.01918045503932	40.0	39.0	41.0	35.0	41.0
28-29	38.915043367769734	40.0	38.0	41.0	35.0	41.0
30-31	38.81851520634219	40.0	38.0	41.0	35.0	41.0
32-33	38.69745916431873	40.0	38.0	41.0	35.0	41.0
34-35	38.68455966981566	40.0	38.0	41.0	35.0	41.0
36-37	38.74652781614276	40.0	38.0	41.0	35.0	41.0
38-39	38.666150350809346	40.0	38.0	41.0	35.0	41.0
40-41	38.55518491151501	40.0	38.0	41.0	34.5	41.0
42-43	38.543935187742846	40.0	38.0	41.0	34.5	41.0
44-45	38.47819618621439	40.0	38.0	41.0	34.0	41.0
46-47	38.31712152183121	40.0	38.0	41.0	34.0	41.0
48-49	38.13983723274957	40.0	37.5	41.0	33.5	41.0
50-51	37.984045540762025	40.0	37.0	41.0	33.0	41.0
52-53	37.813583848958615	40.0	37.0	41.0	33.0	41.0
54-55	37.623384642653264	40.0	36.0	41.0	33.0	41.0
56-57	37.416816980645635	39.0	36.0	41.0	33.0	41.0
58-59	37.18762326667035	39.0	35.0	41.0	32.0	41.0
60-61	36.92816269358967	39.0	35.0	41.0	32.0	41.0
62-63	36.64354455554941	38.0	35.0	40.0	31.5	41.0
64-65	36.3512983859087	37.5	35.0	40.0	31.0	41.0
66-67	36.02123823729812	37.0	35.0	40.0	31.0	41.0
68-69	35.66261072132295	36.5	35.0	39.0	31.0	41.0
70-71	35.32735046866655	36.0	34.5	39.0	30.5	40.5
72-73	34.96426244866766	35.5	34.0	38.5	30.0	40.0
74-75	34.60446660405503	35.0	34.0	37.0	30.0	39.5
76-77	33.36847388726221	34.5	32.5	36.0	28.0	39.0
78-79	33.98392526655986	35.0	34.0	36.0	29.5	39.0
80-81	33.90720229545329	35.0	34.0	36.0	30.0	37.5
82-83	33.69584484282636	35.0	34.0	36.0	30.0	37.0
84-85	33.48095040789643	35.0	34.0	35.0	29.0	37.0
86-87	33.31201107673609	35.0	34.0	35.0	29.0	36.0
88-89	33.15528953280666	35.0	34.0	35.0	29.0	36.0
90-91	33.002427467174925	35.0	33.0	35.0	29.0	36.0
92-93	32.8962453731838	35.0	33.0	35.0	29.0	35.0
94-95	32.803620426127466	35.0	33.0	35.0	29.0	35.0
96-97	32.711436637938974	35.0	33.0	35.0	29.0	35.0
98-99	32.64479529215697	35.0	33.0	35.0	29.0	35.0
100	31.375840883560763	34.0	31.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1569576425037127
1101	2	0.09151892671321349
1101	3	0.01800459656465847
1101	4	0.008568811903693074
1101	5	0.0015199831621828253
1101	6	0.02616905367080591
1101	7	0.018759364177562077
1101	8	0.04610080690668639
1101	9	0.012734599132024016
1101	10-11	0.03843914146408878
1101	12-13	0.030468000200677636
1101	14-15	0.018333476611147148
1101	16-17	-0.02837331523850395
1101	18-19	-0.04339973602494496
1101	20-21	0.003856255199458758
1101	22-23	-0.08696955737138268
1101	24-25	-0.034535858282616516
1101	26-27	-0.02693327363135012
1101	28-29	-0.011173455087103434
1101	30-31	-0.022537483564590843
1101	32-33	-0.026869976878700186
1101	34-35	-0.023235311982823248
1101	36-37	0.08076673429333425
1101	38-39	0.05694444641299867
1101	40-41	0.07845013326391381
1101	42-43	0.038976825731459996
1101	44-45	-0.004107823699250446
1101	46-47	0.14556904423876205
1101	48-49	0.0828444417435108
1101	50-51	-0.0028169152698609423
1101	52-53	0.05712175455587243
1101	54-55	0.0735366376455957
1101	56-57	0.09178713811555639
1101	58-59	0.04104358641433237
1101	60-61	0.023488950336073344
1101	62-63	-0.004140322736667201
1101	64-65	-0.017415189226703376
1101	66-67	-0.03050519424490261
1101	68-69	-0.012706146857816236
1101	70-71	-0.02771961450408611
1101	72-73	-0.04207636602421161
1101	74-75	0.009846683772387621
1101	76-77	0.06911313032559008
1101	78-79	0.09853336452239603
1101	80-81	0.05983967768364806
1101	82-83	0.09254167999664276
1101	84-85	0.10629710781741863
1101	86-87	0.07705168324440592
1101	88-89	0.044159138737676074
1101	90-91	0.08245351846939997
1101	92-93	0.02923977073102435
1101	94-95	0.029845719547310523
1101	96-97	0.05882529989929708
1101	98-99	-0.001245651073730869
1101	100	0.14255422521071637
1102	1	0.14921399377524835
1102	2	0.06465843375337954
1102	3	0.03311065573642935
1102	4	0.046723825448339085
1102	5	-0.012762917912894522
1102	6	0.026667539218209413
1102	7	0.03150918606409192
1102	8	0.037355438094394344
1102	9	0.04054193496923375
1102	10-11	0.041763592077295186
1102	12-13	-0.006851323192918812
1102	14-15	0.05506611517793658
1102	16-17	-0.025455035102822876
1102	18-19	-0.018573947410466474
1102	20-21	0.00468540229897485
1102	22-23	-0.012099845885124694
1102	24-25	0.015182653308237093
1102	26-27	-0.04702685298764919
1102	28-29	0.02444908091314346
1102	30-31	0.0378644856406396
1102	32-33	-0.06870266028649752
1102	34-35	-0.02327325112843681
1102	36-37	0.04235313829658338
1102	38-39	0.011072853377292802
1102	40-41	0.0531605003386062
1102	42-43	0.039617015834544134
1102	44-45	0.09532811776830385
1102	46-47	0.016190621470890676
1102	48-49	-0.005320565784693088
1102	50-51	0.04684537356766327
1102	52-53	0.059499916340421066
1102	54-55	0.02434423488826809
1102	56-57	-0.041103241181978944
1102	58-59	-0.05235702320811697
1102	60-61	5.535423589719812E-5
1102	62-63	0.05836996014372886
1102	64-65	-0.022527396756260032
1102	66-67	-0.06406094384063721
1102	68-69	-0.01593187126595552
1102	70-71	-0.0732391755370898
1102	72-73	-0.08386301062180479
1102	74-75	-0.03842164487158328
1102	76-77	-0.05450527004394701
1102	78-79	0.027892126475727252
1102	80-81	0.00994877779137937
1102	82-83	0.07655167566872478
1102	84-85	-7.006488657026466E-4
1102	86-87	0.03843643989723944
1102	88-89	0.077087467734124
1102	90-91	-0.016263109461341685
1102	92-93	-0.011008438873730597
1102	94-95	0.0043026489079949215
1102	96-97	-0.07001495687958936
1102	98-99	-0.049805330194033104
1102	100	0.1069260959567444
1103	1	0.14617058037357822
1103	2	0.10329605368013262
1103	3	0.06027154463387063
1103	4	0.016553992676278995
1103	5	0.030808708822824826
1103	6	0.035477197129864635
1103	7	0.025621853610054757
1103	8	0.029597702274706705
1103	9	-0.02090629985622172
1103	10-11	0.04988001497100214
1103	12-13	-0.024690297254259974
1103	14-15	0.038257173228181784
1103	16-17	0.07470357702744224
1103	18-19	0.06860105568603814
1103	20-21	-0.00918034417603053
1103	22-23	-0.007198870193519724
1103	24-25	-0.010779255719832292
1103	26-27	-0.025184627806545734
1103	28-29	0.021019258359544324
1103	30-31	0.006732173707220568
1103	32-33	-0.036434318522871934
1103	34-35	-0.044221831590668614
1103	36-37	0.01605464139810664
1103	38-39	0.0754634206316851
1103	40-41	0.052522291030008716
1103	42-43	-0.037070359049955925
1103	44-45	0.019990625007423546
1103	46-47	0.08019925171988262
1103	48-49	0.038941340690847426
1103	50-51	0.0926108366288858
1103	52-53	-0.03627777058797932
1103	54-55	0.017814011755881154
1103	56-57	0.04999514206643596
1103	58-59	0.005527433891082012
1103	60-61	0.03875804258701976
1103	62-63	-0.0700867475948499
1103	64-65	-0.0016721037014661988
1103	66-67	-0.013266198942332608
1103	68-69	0.015126297185524606
1103	70-71	0.0318419361245148
1103	72-73	-0.05153545868422782
1103	74-75	0.005685567178673523
1103	76-77	-0.01800123015260624
1103	78-79	0.03659785274814453
1103	80-81	0.05463768272537095
1103	82-83	0.0434975176081025
1103	84-85	0.037733946069756996
1103	86-87	0.03253498487521256
1103	88-89	0.10312477558350963
1103	90-91	0.09797188463019069
1103	92-93	0.02245605824568031
1103	94-95	-0.03767890743665703
1103	96-97	-0.06998880397590312
1103	98-99	-0.00508430949432892
1103	100	0.03962018607002449
1104	1	0.0805332416193707
1104	2	0.05931130550298036
1104	3	0.030386863649397355
1104	4	-0.005381287387450584
1104	5	0.026393524192648954
1104	6	0.012927630056935868
1104	7	-0.06396415467926175
1104	8	-0.017155313495614166
1104	9	4.882891374862197E-4
1104	10-11	-0.026180062807846127
1104	12-13	-0.0713203152915014
1104	14-15	-0.014561270579449115
1104	16-17	-0.013154693825569552
1104	18-19	-0.04845791129309163
1104	20-21	-0.04695491524060458
1104	22-23	-0.09421465984883781
1104	24-25	-0.045955074131619256
1104	26-27	-0.12898936114807924
1104	28-29	-0.07549274630235914
1104	30-31	-0.1725522118124303
1104	32-33	-0.11070888917164012
1104	34-35	-0.12962149911997756
1104	36-37	-0.024991024882147883
1104	38-39	-0.07471712330467284
1104	40-41	-0.07152285883127973
1104	42-43	-0.14282257815463595
1104	44-45	-0.1704801613547886
1104	46-47	-0.060661738648619234
1104	48-49	-0.12301868548768624
1104	50-51	-0.11664702836539931
1104	52-53	-0.15908496502762404
1104	54-55	-0.14725778170647175
1104	56-57	-0.01521727889069524
1104	58-59	-0.07637801175103931
1104	60-61	-0.16006819573742348
1104	62-63	-0.10707533377944145
1104	64-65	-0.14129173431637554
1104	66-67	-0.1300356677709189
1104	68-69	-0.07448624410304916
1104	70-71	-0.05130816965405671
1104	72-73	-0.07444734022886479
1104	74-75	-0.03518172250326046
1104	76-77	-0.18180367557518906
1104	78-79	-0.08696073751956135
1104	80-81	-0.05352108720789772
1104	82-83	-0.04652307574147585
1104	84-85	-0.042885597643739004
1104	86-87	-0.10819947349194337
1104	88-89	-0.045590892118759996
1104	90-91	0.016439901997173934
1104	92-93	-0.05934122839262557
1104	94-95	-0.04532355568133539
1104	96-97	-0.1256208891442867
1104	98-99	-0.16628943156379705
1104	100	-0.07894053307456517
1105	1	0.1287606438899047
1105	2	0.09586332074242421
1105	3	0.04169842158642467
1105	4	0.013055150021877182
1105	5	-0.021554191199868455
1105	6	0.026305421009062968
1105	7	0.0382521071413322
1105	8	0.05818015417852962
1105	9	0.052911405278486257
1105	10-11	0.02316536824928761
1105	12-13	0.031086177756421307
1105	14-15	0.11146084686173197
1105	16-17	0.14845098196990136
1105	18-19	0.06534148401238582
1105	20-21	0.03422366205877836
1105	22-23	0.03209001937218403
1105	24-25	0.013429616790254784
1105	26-27	0.07253712444973814
1105	28-29	0.12028871002638652
1105	30-31	0.08660257937061999
1105	32-33	0.08217743833943558
1105	34-35	0.015159301423132376
1105	36-37	0.031499666647768265
1105	38-39	0.13727569909220705
1105	40-41	0.11205805317588613
1105	42-43	0.15237436956208938
1105	44-45	0.1276720057616103
1105	46-47	0.1066805727293314
1105	48-49	0.1506521010844324
1105	50-51	0.11904887191165159
1105	52-53	0.04785403664013188
1105	54-55	0.1361399785191182
1105	56-57	0.10417546894355922
1105	58-59	0.07911906236896016
1105	60-61	0.05213708703843167
1105	62-63	0.07096125304264689
1105	64-65	0.12529259892662736
1105	66-67	0.11719541673548406
1105	68-69	0.16534450939948186
1105	70-71	0.11541947744516534
1105	72-73	0.03158951211094774
1105	74-75	8.93315720098542E-4
1105	76-77	-0.04941660248912427
1105	78-79	0.1255719247021645
1105	80-81	0.1016678333722183
1105	82-83	0.1599527587064813
1105	84-85	0.13734572003774304
1105	86-87	0.0995940670759552
1105	88-89	0.1262355987257493
1105	90-91	0.1702483049861243
1105	92-93	0.13221484097177694
1105	94-95	0.10816129250793693
1105	96-97	0.07722063302176707
1105	98-99	-0.045526998357772186
1105	100	0.10956606118016765
1106	1	0.0746000867979646
1106	2	0.07180663604226112
1106	3	0.023646387961427706
1106	4	0.0570448982922116
1106	5	0.005371892111533327
1106	6	0.030546321667223708
1106	7	0.009749130692235042
1106	8	0.05760874563114271
1106	9	0.03576195814309813
1106	10-11	0.0437459505269544
1106	12-13	0.04622566848779286
1106	14-15	0.006337726651025832
1106	16-17	0.029361875253023584
1106	18-19	0.01672268845896241
1106	20-21	-0.020166896594858486
1106	22-23	-0.021284471175881947
1106	24-25	0.0014108958176919373
1106	26-27	-0.017676962340757996
1106	28-29	0.04369662017609244
1106	30-31	0.01553351469208053
1106	32-33	-0.01890146242527635
1106	34-35	-0.004605236123836676
1106	36-37	0.009520693104931865
1106	38-39	-0.008511008539244358
1106	40-41	0.00680813333714525
1106	42-43	0.032777557944818625
1106	44-45	-0.0034664077805928173
1106	46-47	-0.06110683250263804
1106	48-49	-9.065722025596301E-4
1106	50-51	0.010579740430365803
1106	52-53	0.010985424348625372
1106	54-55	0.03199727667831098
1106	56-57	0.021913298761845112
1106	58-59	0.041072135163489065
1106	60-61	0.019054739997571346
1106	62-63	-0.04075347291114895
1106	64-65	0.01589546026354327
1106	66-67	-0.0037722386239309458
1106	68-69	0.017580507408766266
1106	70-71	0.026518354215177453
1106	72-73	-0.0063244881681825404
1106	74-75	0.06212983895532176
1106	76-77	-0.010423995150510734
1106	78-79	0.09229623861185843
1106	80-81	0.055938894516984305
1106	82-83	0.10707376910100663
1106	84-85	0.14300536877036052
1106	86-87	0.14395226346670142
1106	88-89	0.14497702936990464
1106	90-91	0.09481310982508973
1106	92-93	0.0745683575244982
1106	94-95	0.083800217806143
1106	96-97	0.12371862377430887
1106	98-99	0.16597538885790186
1106	100	0.13624371039608718
1107	1	0.020261520411210654
1107	2	-0.041543735139434546
1107	3	-0.029568491759476956
1107	4	-0.0028201511310754768
1107	5	-0.019281604027902688
1107	6	0.015186566146830671
1107	7	-0.07257477773608656
1107	8	-0.0247056973258708
1107	9	-0.0841236448750351
1107	10-11	-0.028434353179456195
1107	12-13	-7.913558384657904E-4
1107	14-15	-0.026295161805158784
1107	16-17	-0.056314838335339346
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2316	10-11	2.846828639420096E-4
2316	12-13	-0.004777332769137388
2316	14-15	0.023549573540321944
2316	16-17	0.054600061966709745
2316	18-19	0.014830072797366256
2316	20-21	0.03212862616097567
2316	22-23	-0.07561668364737528
2316	24-25	0.006724484144953635
2316	26-27	0.055827604720995794
2316	28-29	0.0734910773842401
2316	30-31	-0.3025548398650244
2316	32-33	-0.09153422423627688
2316	34-35	-0.12840293957197702
2316	36-37	-0.3999326230424245
2316	38-39	-0.29933920851974705
2316	40-41	-0.18769005301635389
2316	42-43	-0.2909554122304243
2316	44-45	-0.13607221002008885
2316	46-47	-0.36848832659059383
2316	48-49	-0.3933851633412573
2316	50-51	-0.3245585921011269
2316	52-53	-0.38174265216841263
2316	54-55	-0.18289646441889573
2316	56-57	-0.9856585217253127
2316	58-59	-0.8077036355826692
2316	60-61	-0.4188672090270842
2316	62-63	-0.1862973538945525
2316	64-65	-0.18509542047772953
2316	66-67	-0.047889486317821195
2316	68-69	-0.15752285286126977
2316	70-71	-0.21501700924735445
2316	72-73	-0.10829990353167318
2316	74-75	-0.11900283466749073
2316	76-77	-0.14376438925594215
2316	78-79	-0.1479010799527387
2316	80-81	-0.10987733502500419
2316	82-83	-0.2549353750276424
2316	84-85	-0.09750139456313178
2316	86-87	-0.1648020076572223
2316	88-89	-0.14699583039430308
2316	90-91	-0.1117131875489008
2316	92-93	-0.2145332406969871
2316	94-95	-0.16566822325827957
2316	96-97	-0.2519939210451554
2316	98-99	-0.12596302462948472
2316	100	-0.2892008252380869
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	6.0
13	32.0
14	115.0
15	373.0
16	848.0
17	1720.0
18	3163.0
19	4915.0
20	7691.0
21	11535.0
22	15770.0
23	17451.0
24	18739.0
25	20540.0
26	24970.0
27	31497.0
28	39622.0
29	49374.0
30	61137.0
31	76082.0
32	97031.0
33	127272.0
34	188480.0
35	289383.0
36	523696.0
37	1113563.0
38	1362976.0
39	256221.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.685656878419266	19.85113307632052	13.822050387540491	23.64115965771973
2	15.894873454610412	20.726175426061157	39.10932523550876	24.269625883819664
3	19.005990108103703	25.582885721112607	30.06252989644493	25.348594274338755
4	13.393496544689762	17.426982242690972	38.702582280718836	30.476938931900428
5	14.092869450317732	37.73287861226435	34.15345800179786	14.02079393562006
6	30.428935786236487	36.969688599156584	18.490852254939874	14.110523359667054
7	27.65354936815156	32.38215769946306	21.928287538574086	18.036005393811298
8	26.702504216509563	34.296065611582065	20.110178252409575	18.891251919498796
9	26.310298645100488	16.245534733888785	20.61554490403735	36.82862171697337
10-11	25.339469893041045	25.833031880285525	27.94769575735575	20.87980246931768
12-13	26.112882423311618	23.225562969976085	27.914211402370544	22.747343204341753
14-15	23.59991842050684	24.907354101268602	25.325897875088998	26.16682960313556
16-17	23.183145122343664	27.737327006485373	25.968052394919432	23.11147547625153
18-19	22.778960327038877	27.291524570846644	27.61458802590032	22.314927076214154
20-21	23.98010876737407	26.330343579079862	27.307583365550432	22.38196428799564
22-23	23.88608619429165	26.03972347701504	27.039347806470744	23.034842522222572
24-25	23.536602489733532	26.2412873137764	27.23957470121356	22.982535495276505
26-27	23.42176042046488	26.513151958130297	26.903677686559192	23.161409934845622
28-29	23.193820893058724	26.545556594216336	27.1678782393353	23.092744273389638
30-31	23.73639720020032	26.238390422496373	27.13216660817733	22.893045769125987
32-33	23.628248733117687	26.482954198159582	26.750610174357405	23.138186894365333
34-35	23.773454595264706	26.53846375206762	26.98135143130441	22.706730221363262
36-37	23.69588499421693	26.307119348612428	26.80386038485056	23.19313527232008
38-39	23.638422007133347	26.336179037026763	27.020863058015866	23.004535897824024
40-41	23.7228287614111	25.867220580930823	27.229709235300177	23.1802414223579
42-43	23.474569696712987	25.94330164727159	27.06758401471833	23.5145446412971
44-45	23.57959113347155	26.268141942997076	26.919058726956674	23.233208196574708
46-47	23.48786746535033	26.160036151171013	26.584151726262157	23.767944657216503
48-49	23.79862618571886	26.072151379719422	26.847578075053068	23.281644359508647
50-51	23.709670680506605	26.193145660160017	26.626285408846424	23.470898250486954
52-53	23.50168042455884	26.28376459760568	26.60592222613695	23.608632751698526
54-55	23.394002064443413	26.168846057161694	26.72623196719727	23.710919911197625
56-57	23.748254294831792	26.12935355907475	26.47128963847013	23.651102507623325
58-59	23.313469718009554	26.290088583475814	26.89479036164824	23.50165133686639
60-61	23.4887100180642	26.434930030713172	26.62914177491856	23.447218176304073
62-63	23.568463861100224	26.196843743504267	26.508949755271228	23.725742640124288
64-65	23.18663549731373	26.111806606679743	26.726922146864183	23.97463574914234
66-67	23.387765169464107	26.121376890618812	26.592386433387478	23.8984715065296
68-69	23.143688142600478	26.16451032591994	26.753035393453057	23.938766138026526
70-71	23.23483942015481	25.898912433468674	26.694986457787106	24.171261688589414
72-73	23.25022072906947	26.146326539984504	26.616202359668854	23.98725037127717
74-75	23.3480674150423	26.309636255187453	26.500006791137082	23.842289538633167
76-77	23.14035004322566	26.207179061724123	26.371518280061974	24.280952614988248
78-79	23.075564935174054	26.13587264405568	26.58257467305039	24.205987747719877
80-81	23.081492370373347	26.47201169647047	26.472552645569113	23.973943287587073
82-83	23.407500267883975	26.122594986017667	26.78717743287852	23.682727313219843
84-85	23.430229674033882	26.193352782349987	26.44496537147357	23.93145217214256
86-87	22.84607121312869	26.48414398149006	26.678007096842588	23.991777708538667
88-89	23.07174725053258	25.95929085937107	26.70548813872971	24.263473751366636
90-91	23.17508778023813	26.027158555964874	26.630453110269613	24.167300553527387
92-93	23.1341308033438	26.130237065666396	26.613000687130178	24.122631443859625
94-95	22.75789786994931	26.060378234977865	26.82038632411305	24.361337570959773
96-97	22.79065532925988	26.012578541068326	26.79242138469381	24.404344744977983
98-99	22.591711625772046	26.127054581432013	26.737878421309848	24.543355371486093
100	22.832738657029076	25.937156142814395	26.518174075182433	24.711931124974104
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	36.0
2	51.0
3	80.5
4	69.5
5	66.5
6	120.0
7	247.5
8	432.5
9	565.0
10	548.5
11	510.5
12	588.5
13	718.5
14	834.0
15	986.5
16	1156.5
17	1383.0
18	1639.5
19	1811.5
20	2039.5
21	2338.5
22	2706.0
23	3192.0
24	3849.0
25	4764.0
26	5819.0
27	7418.0
28	9540.0
29	12362.0
30	16608.0
31	22077.0
32	29872.0
33	40245.5
34	51820.5
35	64726.0
36	81664.0
37	102931.0
38	120123.0
39	131804.5
40	138674.5
41	142294.5
42	149702.0
43	159839.5
44	171238.0
45	187025.0
46	199753.5
47	208200.0
48	215366.0
49	215903.5
50	209027.0
51	201691.0
52	194404.0
53	185913.5
54	176734.5
55	158830.5
56	138859.5
57	117919.5
58	98025.5
59	84740.0
60	69081.5
61	52729.0
62	40464.0
63	30634.0
64	21693.0
65	14172.5
66	9584.5
67	6886.0
68	5030.0
69	3590.5
70	2573.5
71	1896.5
72	1350.5
73	962.0
74	695.5
75	431.0
76	251.5
77	134.5
78	71.0
79	39.0
80	21.0
81	12.0
82	9.0
83	7.5
84	4.0
85	1.5
86	2.5
87	3.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3515689740898293
2	6.445316096716572E-4
3	0.0017954809126567593
4	0.0013120822054030162
5	0.004258512421044878
6	0.0
7	4.6037972119404085E-5
8	1.611329024179143E-4
9	0.0035449238531941145
10-11	8.056645120895715E-5
12-13	6.905695817910614E-5
14-15	2.532088466567225E-4
16-17	4.6037972119404085E-5
18-19	0.001081892344805996
20-21	3.337752978656796E-4
22-23	1.8415188847761634E-4
24-25	0.0
26-27	0.0022098226617313964
28-29	0.011636097453179383
30-31	0.003303224499567243
32-33	0.013914977073089883
34-35	0.01093401837835847
36-37	0.012154024639522677
38-39	0.012833084728283888
40-41	0.01398403403126899
42-43	0.006192107250059849
44-45	0.0013696296705522715
46-47	0.005961917389462829
48-49	0.01305176509585106
50-51	0.003349262471686647
52-53	0.0057202180358359575
54-55	0.004880025044656833
56-57	1.611329024179143E-4
58-59	7.481170469403164E-4
60-61	2.8773732574627553E-4
62-63	9.898164005671878E-4
64-65	3.5679428392538165E-4
66-67	0.0013926486566119736
68-69	0.001726423954477653
70-71	0.0010588733587462938
72-73	0.002612654917776182
74-75	0.0078955122184778
76-77	0.00406285103953741
78-79	0.0015883100381194409
80-81	5.40946172402998E-4
82-83	1.9566138150746735E-4
84-85	0.0014156676426716757
86-87	0.004500211774671749
88-89	0.0018530283778060144
90-91	2.4169935362687147E-4
92-93	0.0019566138150746734
94-95	0.003257186527447839
96-97	0.0021983131687015453
98-99	0.0024630315083881186
100	0.003222658048358286
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4344240.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.90908617478513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.13610075914016	51.49878998295863
2	13.505082856148409	17.802153395269972
3	4.004085923249769	7.917169325001392
4	1.6915510891820609	4.459543460238083
5	0.831583386242017	2.7404450532672358
6	0.46694185644976133	1.8465426633216875
7	0.29901054207275124	1.3795258109249522
8	0.20191061769573387	1.0646195441049773
9	0.15509532436951518	0.9199971989258964
>10	0.6701608646935473	7.486108503426479
>50	0.027368737660546292	1.2188130704951234
>100	0.010631842725740671	1.2097575043343078
>500	3.1746693338426964E-4	0.13778424162649847
>1k	1.5873343654527536E-4	0.31875024610469826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4811	0.11074434193322652	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.3018986059702043E-5	0.0
3	0.0	0.0	0.0	6.905695817910612E-5	0.0
4	0.0	0.0	0.0	1.1509493029851022E-4	0.0
5	0.0	0.0	0.0	1.1509493029851022E-4	0.0
6	0.0	0.0	0.0	1.1509493029851022E-4	0.0
7	0.0	0.0	0.0	1.611329024179143E-4	0.0
8	0.0	0.0	0.0	1.8415188847761634E-4	0.0
9	0.0	0.0	0.0	3.222658048358286E-4	0.0
10-11	0.0	0.0	0.0	3.222658048358286E-4	0.0
12-13	0.0	0.0	0.0	4.0283225604478574E-4	0.0
14-15	0.0	0.0	0.0	4.7188921422389185E-4	4.6037972119404085E-5
16-17	0.0	0.0	0.0	5.06417693313445E-4	4.6037972119404085E-5
18-19	0.0	0.0	0.0	5.639651584627E-4	6.905695817910612E-5
20-21	0.0	0.0	0.0	7.826455260298694E-4	6.905695817910612E-5
22-23	0.0	0.0	0.0	9.552879214776348E-4	8.056645120895715E-5
24-25	0.0	0.0	0.0	0.0011854777820746551	9.207594423880817E-5
26-27	0.0	0.0	0.0	0.0018415188847761635	9.207594423880817E-5
28-29	0.0	0.0	0.0	0.0033837909507762	9.207594423880817E-5
30-31	0.0	0.0	0.0	0.008505515349059904	1.035854372686592E-4
32-33	0.0	0.0	0.0	0.020567464044343772	1.2660442332836124E-4
34-35	0.0	0.0	0.0	0.04321814632709059	1.3811391635821225E-4
36-37	0.0	0.0	0.0	0.08068154613925566	1.611329024179143E-4
38-39	0.0	0.0	0.0	0.1381714638233615	1.8415188847761634E-4
40-41	0.0	0.0	0.0	0.20048385908697494	1.8415188847761634E-4
42-43	0.0	0.0	0.0	0.2668360864040661	1.9566138150746735E-4
44-45	0.0	0.0	0.0	0.3543772903891129	2.1868036756716942E-4
46-47	0.0	0.0	0.0	0.45816989853230944	2.4169935362687144E-4
48-49	0.0	0.0	0.0	0.572228974458133	2.762278327164245E-4
50-51	0.0	0.0	0.0	0.6931361987367181	2.762278327164245E-4
52-53	0.0	0.0	0.0	0.8173811759939598	3.1075631180597755E-4
54-55	0.0	0.0	0.0	0.9581192567629782	3.683037769552327E-4
56-57	0.0	0.0	0.0	1.1181702668360864	3.683037769552327E-4
58-59	0.0	0.0	0.0	1.3041291457193893	3.7981326998508366E-4
60-61	0.0	0.0	0.0	1.4999631696223044	4.0283225604478574E-4
62-63	0.0	0.0	0.0	1.7022194906358765	4.143417490746368E-4
64-65	0.0	0.0	0.0	1.9106564094064784	4.143417490746368E-4
66-67	1.1509493029851021E-5	0.0	0.0	2.147372152551424	4.258512421044878E-4
68-69	2.3018986059702043E-5	0.0	0.0	2.4143578623648785	4.6037972119404087E-4
70-71	2.3018986059702043E-5	0.0	0.0	2.705709168922527	4.6037972119404087E-4
72-73	2.3018986059702043E-5	0.0	0.0	3.0012844594221315	4.833987072537429E-4
74-75	2.3018986059702043E-5	0.0	0.0	3.300289578844631	4.833987072537429E-4
76-77	2.3018986059702043E-5	0.0	0.0	3.6312910888901166	5.06417693313445E-4
78-79	2.3018986059702043E-5	0.0	0.0	3.9968786254903046	5.294366793731469E-4
80-81	2.3018986059702043E-5	0.0	0.0	4.385231939303537	5.294366793731469E-4
82-83	2.3018986059702043E-5	0.0	0.0	4.786464375817173	5.294366793731469E-4
84-85	2.3018986059702043E-5	0.0	0.0	5.197836675690109	5.294366793731469E-4
86-87	2.3018986059702043E-5	0.0	0.0	5.628452847908955	5.294366793731469E-4
88	2.3018986059702043E-5	0.0	0.0	5.964725705762112	5.294366793731469E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5760	0.0	45.67637	1
GTATCAA	9785	0.0	39.801464	1
CAACGCA	11170	0.0	33.618744	5
ATCAACG	11430	0.0	32.73103	3
TATCAAC	12345	0.0	30.988543	2
AACGCAG	12710	0.0	30.395142	6
TCAACGC	12495	0.0	30.354643	4
ACGCAGA	14105	0.0	26.456066	7
GTACTAG	1785	0.0	26.150309	1
CGCAGAG	14565	0.0	25.297838	8
GTACATG	16715	0.0	23.074207	1
TACATGG	16985	0.0	22.052643	2
CGTTAAC	805	0.0	21.671333	1
GCAGAGT	16550	0.0	21.638634	9
ACATGGG	17185	0.0	21.11352	3
GTGGTAT	2665	0.0	20.876833	1
CATGGGG	13175	0.0	19.72706	4
TAGGCAT	2960	0.0	19.212397	5
AGAGTAC	15170	0.0	18.370224	10-11
GAGTACT	6580	0.0	18.24811	12-13
>>END_MODULE