FastQCFastQC Report
Thu 26 May 2016
ERR580259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9920517
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177550.17897252733904895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131180.13223101175069807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130450.042.3236541
GTATCAA238950.041.606111
CAACGCA259450.036.2354665
ATCAACG268300.035.075083
GTACTAG84750.033.127891
TATCAAC300100.032.7121932
AACGCAG301700.032.0016756
TAGTACT108450.030.4281834
CTGTGCG108700.030.172439
GTACATG416800.029.6183431
TAGGCAT103400.029.4131285
TCAACGC328950.029.1091564
ACGCAGA323800.028.8894567
GCATCGT89500.028.8198748
GGGCACC98250.028.8022194
TACATGG425150.028.6861952
ACATGGG418750.028.2317943
CGCAGAG333800.027.9956918
CTAGGCA111650.027.7456654
CTAGTAC130200.026.6079753
Produced by FastQC (version 0.11.5)