FastQCFastQC Report
Thu 26 May 2016
ERR580259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9920517
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158910.16018318400139833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123400.12438867853358851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132050.042.405091
GTATCAA236400.040.154431
CAACGCA261650.034.507495
ATCAACG270950.033.5839423
GTACTAG88350.032.6210981
TATCAAC301200.031.298522
AACGCAG306300.030.6417586
TAGGCAT104350.030.1779025
GTACATG414650.029.1971321
CTAGGCA115800.028.290014
GGGCACC96000.028.19915294
TACATGG424200.028.1975122
CTGTGCG108750.028.1759829
TCAACGC326150.028.086874
TAGTACT109000.027.7695144
ACGCAGA326400.027.5884917
ACATGGG419750.027.5012933
CGCAGAG337700.026.5264328
CTAACGC37250.025.866823
TCTAACG38100.025.7920362
Produced by FastQC (version 0.11.5)