FastQCFastQC Report
Thu 26 May 2016
ERR580258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834642
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55490.19575664228498696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43050.1518710299219443No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG28700.043.9093481
GGTATCA42400.043.3625641
GCATCGT30550.038.7588848
GTATCAA75150.038.7316251
GGGCACC33650.034.78774694
TAGGCAT41650.033.9723135
CAACGCA81100.032.923135
CTAGGCA43300.032.7851684
ATCAACG85650.031.226833
TATCAAC96050.030.2479152
TAGTACT44600.029.4054374
TAACGCC11100.028.79684
CTGTGCG44150.028.7388049
GTACATG126250.028.4182431
CGCCTAC11600.028.3544987
TCTAACG11700.028.1265542
AACGCAG101500.027.9605166
CTAACGC11550.027.6738723
TACATGG128300.027.5913312
TACTAGG47400.027.472982
Produced by FastQC (version 0.11.5)