FastQCFastQC Report
Thu 26 May 2016
ERR580258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834642
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41720.14717907940403058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35520.12530682886939515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG29150.039.3548931
GGTATCA46550.036.3605351
GTATCAA77200.035.992961
CAACGCA79300.032.4794925
TAGGCAT41150.032.209295
ATCAACG83300.030.8075493
GGGCACC24750.030.38143794
CTAGGCA44750.030.2488574
GCATCGT37950.029.2266568
TATCAAC97900.028.238532
CTGTGCG31850.027.8874159
AACGCAG101100.027.2411426
ACGCAGA99800.025.8064867
TAGTACT33900.025.511014
CGCAGAG101800.025.2533178
GTACATG123800.025.2170851
TATCCGC1501.1600514E-625.07531
CGACGAG22100.024.77449624-25
CAATGCG22700.024.74055518-19
TACATGG126500.024.6416872
Produced by FastQC (version 0.11.5)