##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR580258_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2834642 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31374014778586 33.0 31.0 34.0 31.0 34.0 2 32.472634992355296 34.0 31.0 34.0 31.0 34.0 3 32.525718944402854 34.0 31.0 34.0 31.0 34.0 4 36.103325569860324 37.0 35.0 37.0 35.0 37.0 5 36.035376248570365 37.0 35.0 37.0 35.0 37.0 6 36.067272692636315 37.0 35.0 37.0 35.0 37.0 7 36.009237498068536 37.0 35.0 37.0 35.0 37.0 8 36.006115763472074 37.0 35.0 37.0 35.0 37.0 9 37.684027118768434 39.0 37.0 39.0 35.0 39.0 10-11 37.615988720974286 39.0 37.0 39.0 35.0 39.0 12-13 37.5668093184254 39.0 37.0 39.0 35.0 39.0 14-15 38.877082890890634 40.0 38.0 41.0 35.0 41.0 16-17 38.86584796245875 40.0 38.0 41.0 35.0 41.0 18-19 38.834283659100514 40.0 38.0 41.0 35.0 41.0 20-21 38.79594125113506 40.0 38.0 41.0 35.0 41.0 22-23 38.70217367836926 40.0 38.0 41.0 34.0 41.0 24-25 38.610989853392425 40.0 38.0 41.0 34.0 41.0 26-27 38.467353020240296 40.0 38.0 41.0 34.0 41.0 28-29 38.364251640947955 40.0 38.0 41.0 34.0 41.0 30-31 38.217019644808765 40.0 38.0 41.0 34.0 41.0 32-33 38.12825023406836 40.0 37.0 41.0 33.5 41.0 34-35 38.04264083436286 40.0 37.0 41.0 33.0 41.0 36-37 37.92993965375522 40.0 37.0 41.0 33.0 41.0 38-39 37.80318449384437 40.0 37.0 41.0 33.0 41.0 40-41 37.685137664650426 40.0 37.0 41.0 33.0 41.0 42-43 37.70242079952248 40.0 37.0 41.0 33.0 41.0 44-45 37.66364059376811 40.0 37.0 41.0 33.0 41.0 46-47 37.48787077874384 40.0 36.0 41.0 32.5 41.0 48-49 37.27862230927221 39.0 36.0 41.0 32.0 41.0 50-51 37.063693581058914 39.0 35.0 41.0 31.5 41.0 52-53 36.86203936863985 39.0 35.0 41.0 31.0 41.0 54-55 36.612841233566705 38.5 35.0 40.0 31.0 41.0 56-57 36.319307341103396 38.0 35.0 40.0 31.0 41.0 58-59 36.022157471737174 37.5 35.0 40.0 30.0 41.0 60-61 35.732943701532676 37.0 34.5 40.0 30.0 41.0 62-63 35.392074201962714 36.5 34.0 39.5 29.5 41.0 64-65 35.067402515026586 36.0 34.0 39.0 29.0 41.0 66-67 34.7317174443898 35.0 34.0 39.0 29.0 40.5 68-69 34.41265845916345 35.0 33.5 38.0 28.5 40.0 70-71 34.12473867952285 35.0 33.0 37.5 28.0 40.0 72-73 33.83574398460193 35.0 33.0 37.0 28.0 39.5 74-75 33.50837389695066 35.0 33.0 36.0 27.0 39.0 76-77 32.04127170203504 34.0 30.5 35.0 25.5 37.5 78-79 33.00803064372856 35.0 32.5 35.0 27.0 37.5 80-81 33.049603089208446 35.0 33.0 35.0 27.0 37.0 82-83 32.85300754028198 35.0 33.0 35.0 27.0 37.0 84-85 32.67213708115522 35.0 33.0 35.0 27.0 36.0 86-87 32.500823384399155 35.0 33.0 35.0 27.0 36.0 88-89 32.35542548230076 35.0 32.5 35.0 26.0 36.0 90-91 32.18818408109384 34.5 32.0 35.0 26.0 35.0 92-93 32.02643808283374 34.0 32.0 35.0 25.0 35.0 94-95 31.89569846915413 34.0 32.0 35.0 25.0 35.0 96-97 31.766112793079337 34.0 31.5 35.0 25.0 35.0 98-99 31.65302267446824 34.0 31.0 35.0 25.0 35.0 100 29.57879125476868 33.0 27.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06815768849636328 1101 2 -0.08287591357768065 1101 3 -0.04910138980327616 1101 4 -0.011915903502327296 1101 5 -0.013161529876406064 1101 6 -0.07929649776892944 1101 7 -0.08252407245619509 1101 8 0.03052676262527143 1101 9 -0.08429057638207382 1101 10-11 -0.10469572791256354 1101 12-13 -0.09545990634581614 1101 14-15 -0.1580740074889917 1101 16-17 -0.1393659538330354 1101 18-19 -0.12191449881358096 1101 20-21 -0.15249554479582628 1101 22-23 -0.14344575512419055 1101 24-25 -0.1299276674125096 1101 26-27 -0.11664900527070898 1101 28-29 -0.10966740071778247 1101 30-31 -0.16278065016712873 1101 32-33 -0.18400118190904635 1101 34-35 -0.15242457492606576 1101 36-37 -0.13273608229418699 1101 38-39 -0.2109789716915671 1101 40-41 -0.25930408566292584 1101 42-43 -0.16279624401849446 1101 44-45 -0.16529538105721286 1101 46-47 -0.33244929138562185 1101 48-49 -0.2660620653748964 1101 50-51 -0.22482506924884404 1101 52-53 -0.23367360142390936 1101 54-55 -0.19848134870114365 1101 56-57 -0.14843499290796558 1101 58-59 -0.1363799534544654 1101 60-61 -0.1787155429425482 1101 62-63 -0.22242911629830786 1101 64-65 -0.3217394785349441 1101 66-67 -0.3176947457677741 1101 68-69 -0.28366584014207774 1101 70-71 -0.3743563489178854 1101 72-73 -0.2816322327401082 1101 74-75 -0.366072297681292 1101 76-77 -0.25565866864531017 1101 78-79 -0.23971434039228967 1101 80-81 -0.1758871180190127 1101 82-83 -0.29827024326612417 1101 84-85 -0.26631487463681225 1101 86-87 -0.2683126765477297 1101 88-89 -0.1575949912823731 1101 90-91 -0.34716196330104765 1101 92-93 -0.2809038656421379 1101 94-95 -0.26498273032555986 1101 96-97 -0.3581064354950598 1101 98-99 -0.40836651556021053 1101 100 -0.41561214311357375 1102 1 -0.06695053934617334 1102 2 -0.13382138974439073 1102 3 -0.03995973050949431 1102 4 -0.0646011128562094 1102 5 -0.023111055293810523 1102 6 -0.0058070307187634285 1102 7 -0.06767340988817239 1102 8 -0.007350564484873701 1102 9 -0.03918461447888433 1102 10-11 0.01013456750107622 1102 12-13 -0.028544349125887436 1102 14-15 -0.04305771066545816 1102 16-17 -0.0703375755979252 1102 18-19 -0.05223186061918739 1102 20-21 -0.00904257742233483 1102 22-23 -0.1009125027221387 1102 24-25 -0.17636524440934664 1102 26-27 -0.10273501480266134 1102 28-29 -0.10650382673789238 1102 30-31 -0.1437542559470586 1102 32-33 -0.13224101956701872 1102 34-35 -0.12729686945805696 1102 36-37 -0.14707121095446496 1102 38-39 -0.2650193563176728 1102 40-41 -0.2713072216467012 1102 42-43 -0.2707695974565141 1102 44-45 -0.2365436861193615 1102 46-47 -0.09314576746491099 1102 48-49 -0.1842060999066817 1102 50-51 -0.1214798958924348 1102 52-53 -0.16999369373021267 1102 54-55 -0.24026455041781247 1102 56-57 -0.05730546214612531 1102 58-59 -0.1711417569489342 1102 60-61 -0.2676654471828215 1102 62-63 -0.21552985900193278 1102 64-65 -0.2423891070591253 1102 66-67 -0.3377143875166553 1102 68-69 -0.3345685359845092 1102 70-71 -0.1858755402333614 1102 72-73 -0.25383819706132016 1102 74-75 -0.2143441275817608 1102 76-77 -0.2636694393669501 1102 78-79 -0.2668835663350322 1102 80-81 -0.1771990479634411 1102 82-83 -0.1052705303283048 1102 84-85 -0.22538588439506668 1102 86-87 -0.29610004717599736 1102 88-89 -0.4106759496360368 1102 90-91 -0.25587634635607515 1102 92-93 -0.2840590695585874 1102 94-95 -0.19256215137757238 1102 96-97 -0.27736399846738635 1102 98-99 -0.3912632209172351 1102 100 -0.2349266535175616 1103 1 0.032667711826121604 1103 2 -6.441983128269158E-5 1103 3 -0.03733230820866851 1103 4 -0.04259407908737245 1103 5 -0.06762633006621854 1103 6 -0.03227101367772178 1103 7 -0.024627720511354312 1103 8 -0.12119358300544292 1103 9 -0.10965664085009763 1103 10-11 -0.028152815811615994 1103 12-13 -0.03266726056160252 1103 14-15 0.0319352712284342 1103 16-17 -0.00860730822314082 1103 18-19 -0.011086029795905006 1103 20-21 -0.026309678709573348 1103 22-23 -0.01955021302435256 1103 24-25 -0.04282469120186505 1103 26-27 -0.05573222804223832 1103 28-29 -0.024032495550748934 1103 30-31 -0.10102679099542655 1103 32-33 -0.07958459110229654 1103 34-35 -0.07416664352926006 1103 36-37 -0.16324463227216057 1103 38-39 -0.17156258062237129 1103 40-41 -0.04676698289856063 1103 42-43 0.1010198449022397 1103 44-45 0.02417082334815035 1103 46-47 -0.06967637725098541 1103 48-49 -0.10639337550800576 1103 50-51 -0.12683669881678128 1103 52-53 -0.17978334934300477 1103 54-55 -0.043992670794303024 1103 56-57 0.020761329742036594 1103 58-59 -0.052320099977087864 1103 60-61 -0.14760989872235086 1103 62-63 -0.1585600061351755 1103 64-65 -0.059983107512138645 1103 66-67 -0.20828078181253318 1103 68-69 -0.2370231863683685 1103 70-71 -0.19674456851782907 1103 72-73 -0.15281361381030933 1103 74-75 -0.16169492806755414 1103 76-77 -0.22323475654920344 1103 78-79 -0.16746200285761148 1103 80-81 -0.27012193554362085 1103 82-83 -0.0938377945078841 1103 84-85 -0.07059703601507294 1103 86-87 -0.11606484218705759 1103 88-89 -0.22333709499094567 1103 90-91 -0.12925630842817526 1103 92-93 -0.15387335179608996 1103 94-95 -0.22011032630853578 1103 96-97 -0.3040037352853915 1103 98-99 -0.301089894780727 1103 100 -0.1767998673643767 1104 1 0.007754219832108333 1104 2 0.04187316308508571 1104 3 -0.06774213111838634 1104 4 -0.0038104046605198505 1104 5 -0.04334712723061784 1104 6 -0.03146345597017586 1104 7 -0.01504183094080247 1104 8 0.021297000164580027 1104 9 0.06728632391367739 1104 10-11 0.04135039028788157 1104 12-13 0.035929126697944014 1104 14-15 -0.16639920413404496 1104 16-17 -0.11857061457428131 1104 18-19 -0.0902341911009259 1104 20-21 -0.08474277824961973 1104 22-23 -0.09085877919694951 1104 24-25 0.015325286125914772 1104 26-27 -0.06636607666361272 1104 28-29 -0.10631829072853094 1104 30-31 -0.11329904456966489 1104 32-33 -0.003262881773686388 1104 34-35 -0.004249416791296312 1104 36-37 0.001733051861101842 1104 38-39 -0.04029587108050947 1104 40-41 0.04076492096222495 1104 42-43 -0.011364100244442454 1104 44-45 0.04425033326894123 1104 46-47 0.06738872570560517 1104 48-49 0.09505369045918854 1104 50-51 0.06152831109390178 1104 52-53 0.06810649226569154 1104 54-55 0.16104035264788052 1104 56-57 -0.012468982461236067 1104 58-59 -0.009114640236006721 1104 60-61 -0.17148370927233714 1104 62-63 0.007691517505669765 1104 64-65 0.003976557253203339 1104 66-67 -0.0761723518977675 1104 68-69 -0.1596279836764296 1104 70-71 0.021227125995267215 1104 72-73 0.06534481790026803 1104 74-75 0.09177879067115668 1104 76-77 0.08817323069599325 1104 78-79 0.12797083460498726 1104 80-81 0.1585532236670275 1104 82-83 0.08232728566424186 1104 84-85 0.07012361243300091 1104 86-87 0.13653600992942927 1104 88-89 0.08431012294749252 1104 90-91 -0.07393586964206378 1104 92-93 0.057442375207877205 1104 94-95 0.00731193994868562 1104 96-97 -0.04627027590796118 1104 98-99 -5.743261456139237E-4 1104 100 0.005629046232002111 1105 1 -0.020135433004860204 1105 2 0.07643482428424875 1105 3 -0.026570842495935665 1105 4 -0.004908623710925042 1105 5 -0.036129678810219445 1105 6 -0.022620302618953758 1105 7 0.07521604871535459 1105 8 0.04436379389964884 1105 9 -0.008697722292254184 1105 10-11 0.01562860613012873 1105 12-13 0.05134907884809792 1105 14-15 0.02660617582877478 1105 16-17 -8.292789721693339E-4 1105 18-19 0.05246155137378139 1105 20-21 -0.03431073782677174 1105 22-23 -0.0035003026757678413 1105 24-25 0.018640911659950632 1105 26-27 -0.011567370517674647 1105 28-29 -0.12088843363854096 1105 30-31 -0.06876711391630153 1105 32-33 -0.04779074980287845 1105 34-35 0.014916209319373763 1105 36-37 -0.04380005354591532 1105 38-39 -0.1435959538007765 1105 40-41 -0.06581900708686561 1105 42-43 -0.0792014710398945 1105 44-45 -0.036108766013207116 1105 46-47 -0.2043204962939953 1105 48-49 -0.19963524096056773 1105 50-51 -0.06704876396347714 1105 52-53 -0.20017629940189607 1105 54-55 -0.059663540237231416 1105 56-57 -0.12467446841923646 1105 58-59 -0.17052263306901239 1105 60-61 -0.162608382558723 1105 62-63 -0.12898053279328536 1105 64-65 -0.08264206298526489 1105 66-67 -0.16506407588674676 1105 68-69 -0.1894189446259702 1105 70-71 -0.1312506946978047 1105 72-73 -0.009544940827034054 1105 74-75 -0.01777419485961218 1105 76-77 -0.10988559505360485 1105 78-79 -0.04978463368714614 1105 80-81 0.05985294887636883 1105 82-83 0.021970188862653117 1105 84-85 0.014276814776849278 1105 86-87 -0.0032886367271132144 1105 88-89 -0.029693336311460428 1105 90-91 0.033616470062511894 1105 92-93 0.10673484955044188 1105 94-95 0.13929021775249595 1105 96-97 -0.04434917888410794 1105 98-99 -0.05164472277305876 1105 100 0.3091525470712604 1106 1 -0.013439478529534199 1106 2 -0.07339285519959304 1106 3 -0.06467421476836677 1106 4 -0.0031313179221612586 1106 5 0.022500874018042794 1106 6 -0.00802715937251719 1106 7 -0.09651036240933308 1106 8 -0.03759765439417606 1106 9 -0.08412251240185498 1106 10-11 -0.12475150086888931 1106 12-13 -0.07344228308509315 1106 14-15 -0.05492522257225119 1106 16-17 -0.06115273059218396 1106 18-19 0.028502487515005726 1106 20-21 -0.06510075726291831 1106 22-23 -0.057885459066653766 1106 24-25 -0.09711890159270098 1106 26-27 -0.10588104395026221 1106 28-29 -0.003555694744200366 1106 30-31 -0.05427741307273237 1106 32-33 -0.10332729778212268 1106 34-35 -0.0693565379906218 1106 36-37 -0.14759911980915774 1106 38-39 0.03289331624149838 1106 40-41 -0.04635405227090672 1106 42-43 -0.07236650800865618 1106 44-45 0.03060181566871023 1106 46-47 -0.03754930187238159 1106 48-49 -0.09423444548097137 1106 50-51 -0.10104649975204438 1106 52-53 -0.01924852373916508 1106 54-55 -0.09518561128938785 1106 56-57 -0.042982020466041604 1106 58-59 -0.11761998414200292 1106 60-61 -0.1646148646153449 1106 62-63 -0.05671361632865768 1106 64-65 -0.14089766841136253 1106 66-67 -0.17049843379245289 1106 68-69 -0.18328804114549513 1106 70-71 -0.0657806704527033 1106 72-73 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-0.14838223621255509 2316 24-25 -0.13176768753217516 2316 26-27 -0.13561247660836528 2316 28-29 -0.21109755275465147 2316 30-31 -0.2506166624455233 2316 32-33 -0.24450331183712848 2316 34-35 -0.20526094926497507 2316 36-37 -0.12838168811227746 2316 38-39 -0.1612973254088601 2316 40-41 -0.16880238657389413 2316 42-43 -0.2887109659183835 2316 44-45 -0.21485089012598024 2316 46-47 -0.26840091490912954 2316 48-49 -0.23005469231733144 2316 50-51 -0.2222156380783673 2316 52-53 -0.24992577151605389 2316 54-55 -0.2975215768693502 2316 56-57 -0.4518986792758284 2316 58-59 -0.3586012817000963 2316 60-61 -0.3463274384604489 2316 62-63 -0.30553738381404827 2316 64-65 -0.2854519303283354 2316 66-67 -0.30256249764705245 2316 68-69 -0.23451313962478082 2316 70-71 -0.2428706965068912 2316 72-73 -0.34135346690190715 2316 74-75 -0.3018922038466272 2316 76-77 -0.24232640635361236 2316 78-79 -0.32111943109299546 2316 80-81 -0.39666065837656106 2316 82-83 -0.28768503407430757 2316 84-85 -0.11260882832992536 2316 86-87 -0.2642172399896836 2316 88-89 -0.342744306858215 2316 90-91 -0.42844052747543415 2316 92-93 -0.317783363335721 2316 94-95 -0.2276025769001464 2316 96-97 -0.2787845553861459 2316 98-99 -0.43577501611835245 2316 100 -0.6433112264386232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 23.0 14 83.0 15 252.0 16 705.0 17 1641.0 18 3180.0 19 4724.0 20 7483.0 21 11171.0 22 14692.0 23 15602.0 24 16091.0 25 18685.0 26 23480.0 27 29073.0 28 36437.0 29 44736.0 30 55821.0 31 70139.0 32 90062.0 33 121083.0 34 181555.0 35 269127.0 36 474689.0 37 729440.0 38 534297.0 39 80363.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.04288577974434 22.74636215269962 13.353649630658746 23.857102436897293 2 15.250532053980676 21.778625315782993 39.286491880773326 23.684350749463007 3 17.789251059671454 28.078691740118995 29.693484630133653 24.438572570075902 4 12.43655198398083 17.84146921404189 39.47158051001127 30.250398291966 5 12.947859781058286 39.182057349336894 34.86743297560411 13.002649894000712 6 29.190221971671193 39.11193692605166 18.11926095756041 13.578580144716737 7 26.17444313523506 32.57223190410002 23.74875644707227 17.50456851359265 8 26.62464623315484 34.72559829020629 19.95770673707547 18.692048739563393 9 26.518521131729344 14.47452903407888 21.0825160516475 37.92443378254428 10-11 25.501492606816107 25.89886257081151 27.74530645535691 20.854338367015472 12-13 26.056024750938388 23.38699094064855 27.820781474896283 22.736202833516778 14-15 23.4740324054075 24.657806806169223 25.10580344204928 26.762357346374 16-17 22.50805525995643 27.39342247697546 26.207221837987454 23.891300425080658 18-19 21.90849719317896 27.466593758406134 28.305368017674375 22.319541030740535 20-21 23.419842879463665 26.904783995422342 27.280246267690522 22.395126857423467 22-23 24.05593939328728 26.179287508817374 27.0896970246456 22.675076073249752 24-25 23.421475970239293 26.339690468882843 27.55800621602266 22.6808273448552 26-27 23.325512647127358 26.7577361203693 26.664706350313704 23.252044882189633 28-29 22.63143161590742 27.161866614784703 27.41346827347887 22.79323349582901 30-31 23.478682370522304 26.64956366713287 27.306995490177506 22.56475847216732 32-33 23.570849251200258 26.960036378992047 26.548091566967223 22.92102280284047 34-35 23.426903079015606 26.969542868744295 27.344125242735057 22.259428809505035 36-37 23.3125655974105 26.3626180753389 26.91780809835863 23.407008228891964 38-39 23.48156520837236 26.260604181240964 27.652399110566417 22.60543149982026 40-41 23.122507647169538 25.546317743003243 28.289667602795582 23.04150700703164 42-43 23.009457417806544 25.81586655365627 27.610138831683013 23.56453719685417 44-45 23.214788125570088 26.445183697063147 27.45693355669668 22.883094620670093 46-47 23.18576986971954 26.163868541937653 26.69470612370029 23.95565546464252 48-49 23.87365034821444 26.069820703830825 27.116430933868095 22.940098014086637 50-51 23.50283342961849 26.010257676720013 26.902737291461786 23.58417160219971 52-53 22.851441674270887 26.330803727430542 26.929339729994663 23.88841486830391 54-55 22.866460129676252 26.409087533499935 26.683260890157612 24.0411914466662 56-57 23.985004289931645 25.80032273268646 26.26831687918762 23.946356098194276 58-59 22.57954466275709 26.46491391316082 27.338456531460213 23.61708489262187 60-61 22.967450130890978 27.009247041722823 26.42265437020625 23.60064845717995 62-63 23.398920324735162 26.097702779553483 26.106716536910895 24.396660358800464 64-65 22.352301796371695 26.23332758285539 26.66360797686544 24.750762643907475 66-67 23.13496272492009 26.10640300903023 26.499165912427596 24.259468353622086 68-69 22.44409675490416 26.012961332964085 26.9982201980184 24.544721714113354 70-71 22.504209643602913 25.66369136971025 26.786651064026444 25.04544792266039 72-73 22.843770252882987 26.01547095350705 26.98129423000325 24.159464563606708 74-75 23.114435534600624 26.390705334346116 26.569647017068256 23.925212113985005 76-77 22.641001212373084 26.245663512756202 26.254853769954632 24.85848150491608 78-79 22.713036195524058 26.41409988156963 26.220737697370716 24.652126225535593 80-81 22.460480428028667 26.904848043437884 26.532817159812055 24.101854368721394 82-83 23.303714452182998 25.768691196836702 27.49630932618148 23.43128502479882 84-85 23.327917493433343 26.21934451462321 26.375593053872233 24.077144938071214 86-87 21.973202142840766 26.94011934853972 26.85587332050357 24.230805188115948 88-89 22.779363916310682 25.649230039790066 26.87521144757317 24.696194596326077 90-91 22.65771584310235 25.78745251313281 26.69509396300959 24.859737680755252 92-93 22.995613020849042 25.904132043645074 26.566967886734055 24.533287048771825 94-95 22.040414078762595 25.880469480238972 27.193608650871386 24.885507790127043 96-97 22.41323533925329 25.816228290975108 26.84900442497388 24.921531944797717 98-99 21.863233061473526 26.240271811594575 26.7846323154451 25.111862811486795 100 22.103433909267522 25.684875086474797 26.132656657403885 26.079034346853796 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 173.0 1 122.5 2 81.0 3 97.5 4 87.0 5 93.0 6 142.5 7 302.0 8 465.0 9 520.5 10 494.0 11 432.5 12 460.5 13 542.5 14 663.0 15 839.0 16 1008.0 17 1173.5 18 1371.0 19 1564.0 20 1811.0 21 2114.5 22 2304.5 23 2549.0 24 2901.5 25 3231.0 26 3853.5 27 4592.5 28 5514.0 29 7459.5 30 10548.0 31 14558.0 32 20401.5 33 27873.5 34 36163.5 35 45611.5 36 57649.0 37 75260.0 38 86478.0 39 88279.5 40 85671.5 41 82075.5 42 84022.0 43 90822.0 44 101028.5 45 119383.5 46 136464.5 47 146546.5 48 153241.5 49 149074.0 50 140383.5 51 138348.5 52 135546.0 53 128230.5 54 121915.0 55 107138.5 56 91707.5 57 74109.0 58 56802.5 59 51470.5 60 41344.5 61 27483.5 62 19569.0 63 15525.0 64 10805.0 65 5471.5 66 3165.5 67 2140.0 68 1538.5 69 1147.0 70 856.0 71 627.0 72 435.0 73 304.0 74 209.0 75 132.0 76 91.5 77 51.0 78 26.0 79 18.0 80 11.0 81 4.5 82 3.0 83 2.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004797783988242607 2 0.028680870459126758 3 0.0013052794673895328 4 0.001340557290832493 5 0.006667508630719505 6 3.5277823442960344E-5 7 7.761121157451277E-4 8 0.005221117869558131 9 0.0014816685846043346 10-11 1.4111129377184138E-4 12-13 7.055564688592069E-5 14-15 7.937510274666079E-4 16-17 1.2347238205036122E-4 18-19 0.0010406957915673302 20-21 1.0583347032888104E-4 22-23 0.0013581962025539733 24-25 0.001128890350174731 26-27 0.0023106974355139027 28-29 8.819455860740086E-5 30-31 0.0018168079073124577 32-33 0.0013758351142754534 34-35 6.526397336947664E-4 36-37 0.00412750534282636 38-39 0.00102305687984585 40-41 0.0034043099622456732 42-43 0.0033337543153597526 44-45 0.0034395877856886335 46-47 0.0022930585237924224 48-49 0.008942928242790448 50-51 0.006332369308011383 52-53 0.005027089840621849 54-55 0.004286255548319681 56-57 0.003457226697410114 58-59 0.005274034604722572 60-61 0.007831676804337197 62-63 0.0030338928160945897 64-65 0.003351393227081233 66-67 0.0037923660201182375 68-69 0.00201083593624874 70-71 0.002786948051993867 72-73 0.005961952161860299 74-75 0.007020286865149108 76-77 0.004180422077990801 78-79 0.0022930585237924224 80-81 0.0023106974355139027 82-83 0.004674311606192246 84-85 0.001957919201084299 86-87 0.0030691706395375496 88-89 5.291673516444052E-4 90-91 0.0014993074963258147 92-93 1.2347238205036122E-4 94-95 0.0021343083182991007 96-97 0.001728613348705057 98-99 0.0011112514384532509 100 3.5277823442960344E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2834642.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.3999974009399 #Duplication Level Percentage of deduplicated Percentage of total 1 77.17145218326546 51.24184224395583 2 14.06080971683188 18.672754573054938 3 4.330173594679622 8.625705462970362 4 1.7850917864503684 4.7412035992297445 5 0.9077066126127918 3.0135858359152667 6 0.5039011124039897 2.007541953237338 7 0.3144795591358439 1.4617009336488134 8 0.205136292602296 1.0896839436504717 9 0.1472602096718077 0.8800269785522905 >10 0.5459609194393713 5.873589205776015 >50 0.020483825409582317 0.925611521721797 >100 0.006865676395190758 0.8100838212448175 >500 4.31779792029388E-4 0.2017561317692122 >1k 2.467313097241298E-4 0.45491379527313003 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4172 0.14717907940403058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3552 0.12530682886939515 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.5277823442960344E-5 0.0 14-15 0.0 0.0 0.0 3.5277823442960344E-5 0.0 16-17 0.0 0.0 0.0 3.5277823442960344E-5 0.0 18-19 0.0 0.0 0.0 3.5277823442960344E-5 0.0 20-21 0.0 0.0 0.0 1.0583347032888103E-4 0.0 22-23 0.0 0.0 0.0 3.5277823442960343E-4 0.0 24-25 0.0 0.0 0.0 8.819455860740086E-4 0.0 26-27 0.0 0.0 0.0 0.00202847484797022 0.0 28-29 0.0 0.0 0.0 0.005520979368823294 1.7638911721480172E-5 30-31 0.0 0.0 0.0 0.01732141131049353 3.5277823442960344E-5 32-33 0.0 0.0 0.0 0.04489103033116704 3.5277823442960344E-5 34-35 0.0 0.0 0.0 0.09122845142349545 3.5277823442960344E-5 36-37 0.0 0.0 0.0 0.16704049400241724 7.055564688592069E-5 38-39 0.0 0.0 0.0 0.2773718868202757 7.055564688592069E-5 40-41 0.0 0.0 0.0 0.4013910751340028 7.055564688592069E-5 42-43 0.0 0.0 0.0 0.5296965189960496 7.055564688592069E-5 44-45 0.0 0.0 0.0 0.6914982562171872 7.055564688592069E-5 46-47 0.0 0.0 0.0 0.8834801713937774 7.055564688592069E-5 48-49 0.0 0.0 0.0 1.0963289191368788 7.055564688592069E-5 50-51 0.0 0.0 0.0 1.3209604599099287 7.055564688592069E-5 52-53 0.0 0.0 0.0 1.5428050526309849 7.055564688592069E-5 54-55 0.0 0.0 0.0 1.785181338595844 7.055564688592069E-5 56-57 1.7638911721480172E-5 0.0 0.0 2.0648462839399118 7.055564688592069E-5 58-59 3.5277823442960344E-5 0.0 0.0 2.3968987970967763 7.055564688592069E-5 60-61 3.5277823442960344E-5 0.0 0.0 2.7430977174542677 7.055564688592069E-5 62-63 3.5277823442960344E-5 0.0 0.0 3.098380677348321 7.055564688592069E-5 64-65 3.5277823442960344E-5 0.0 0.0 3.459255172258084 7.055564688592069E-5 66-67 3.5277823442960344E-5 0.0 0.0 3.848845815450417 7.055564688592069E-5 68-69 3.5277823442960344E-5 0.0 0.0 4.277612481576156 7.055564688592069E-5 70-71 3.5277823442960344E-5 0.0 0.0 4.761888802889395 7.055564688592069E-5 72-73 3.5277823442960344E-5 0.0 0.0 5.249199017018728 7.055564688592069E-5 74-75 3.5277823442960344E-5 0.0 0.0 5.717829623635013 7.055564688592069E-5 76-77 3.5277823442960344E-5 0.0 0.0 6.233220985224943 7.055564688592069E-5 78-79 3.5277823442960344E-5 0.0 0.0 6.776940439039568 7.055564688592069E-5 80-81 3.5277823442960344E-5 0.0 0.0 7.362181891046559 7.055564688592069E-5 82-83 3.5277823442960344E-5 0.0 0.0 7.96876642623654 7.055564688592069E-5 84-85 3.5277823442960344E-5 0.0 0.0 8.552014681219003 7.055564688592069E-5 86-87 3.5277823442960344E-5 0.0 0.0 9.15388962697935 7.055564688592069E-5 88 3.5277823442960344E-5 0.0 0.0 9.625942182469602 7.055564688592069E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2915 0.0 39.354893 1 GGTATCA 4655 0.0 36.360535 1 GTATCAA 7720 0.0 35.99296 1 CAACGCA 7930 0.0 32.479492 5 TAGGCAT 4115 0.0 32.20929 5 ATCAACG 8330 0.0 30.807549 3 GGGCACC 2475 0.0 30.381437 94 CTAGGCA 4475 0.0 30.248857 4 GCATCGT 3795 0.0 29.226656 8 TATCAAC 9790 0.0 28.23853 2 CTGTGCG 3185 0.0 27.887415 9 AACGCAG 10110 0.0 27.241142 6 ACGCAGA 9980 0.0 25.806486 7 TAGTACT 3390 0.0 25.51101 4 CGCAGAG 10180 0.0 25.253317 8 GTACATG 12380 0.0 25.217085 1 TATCCGC 150 1.1600514E-6 25.0753 1 CGACGAG 2210 0.0 24.774496 24-25 CAATGCG 2270 0.0 24.740555 18-19 TACATGG 12650 0.0 24.641687 2 >>END_MODULE