FastQCFastQC Report
Thu 26 May 2016
ERR580257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7475103
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190010.25419047737536193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141030.18866629663832057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89400.11959701424849932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106450.045.724851
GTATCAA189850.040.5009041
CAACGCA210800.034.976385
GTACTAG57950.034.7334481
ATCAACG220000.033.538923
TATCAAC239350.031.9089892
GGGCACC69250.031.6253994
AACGCAG244250.031.1947946
CTGTGCG81600.030.6432519
GCATCGT60650.030.1453638
TAGTACT83600.029.643734
TCAACGC255400.029.2755264
TAGGCAT73150.029.1194575
GTACATG274200.028.6250651
ACGCAGA259400.028.3751437
CGCAGAG266000.027.5111478
TACATGG281300.027.4179252
CTAGTAC94550.027.2066233
ACATGGG278900.026.8100383
CTAACGC28250.026.6348553
Produced by FastQC (version 0.11.5)