FastQCFastQC Report
Thu 26 May 2016
ERR580257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7475103
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164330.21983643569861178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128710.17218491838841551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86720.11601177936946153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112700.045.636541
GTATCAA191800.038.5811731
CAACGCA213800.032.974265
GGGCACC71400.032.52403694
GTACTAG59200.031.8450531
ATCAACG223650.031.5014113
CTGTGCG85050.030.2821089
TAGTACT88850.029.9403084
TATCAAC246400.029.7200952
AACGCAG246400.029.2595356
TCAACGC256900.027.7718584
CTAGTAC100100.027.6553083
TAGGCAT75100.027.473635
GTACATG272550.027.3747431
CTAACGC27500.027.0036013
TAACGCC28050.026.8092354
TACATGG277300.026.7642862
GTACTGT104800.026.6383866
ACGCAGA267400.026.328757
ACTGTGC109450.026.1076418
Produced by FastQC (version 0.11.5)