FastQCFastQC Report
Thu 26 May 2016
ERR580256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6311423
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119450.18926001315392743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98150.15551168096323126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104700.048.728941
GTATCAA186200.039.850271
CAACGCA207800.034.3459135
ATCAACG211050.033.8194273
TATCAAC231150.031.9381452
AACGCAG236800.030.9000746
GTACTAG42850.028.860831
TCAACGC249300.028.7998164
ACGCAGA254950.027.8154967
CGCAGAG258250.027.3684238
GTGGTAT40750.025.6170621
GCATCGT44550.025.5275978
GCAGAGT280950.024.6580629
TAGGCAT58750.024.4084845
CGTTAAC13350.023.9514391
GTACATG306300.023.4881251
TACATGG311500.022.8092162
GTATAAG47550.022.7447551
CTGTGCG55400.022.4815679
TAGTACT56100.022.4618174
Produced by FastQC (version 0.11.5)