FastQCFastQC Report
Thu 26 May 2016
ERR580256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6311423
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104050.16485981053717996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93890.14876201452509205No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100700.051.1456381
GTATCAA183600.040.357011
CAACGCA208550.033.9613235
ATCAACG216050.032.8481643
TATCAAC228700.031.8933542
AACGCAG239400.030.2899976
GTACTAG43700.030.1119021
TCAACGC251200.028.551134
ACGCAGA258200.027.2654727
CGCAGAG265350.026.4778678
GTGGTAT38450.024.9009061
GCAGAGT288000.023.8896319
GGGCTAT58500.023.377316
GTGATCG30400.023.3434078
GTACATG297000.023.2487751
AGGGCTA58650.023.2386825
TAGGCAT61900.022.8537455
GCTATTG56600.022.7507138
TACATGG305200.022.4516162
CTGTGCG58000.022.120539
Produced by FastQC (version 0.11.5)