FastQCFastQC Report
Thu 26 May 2016
ERR580255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7843084
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206780.26364629015831015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166460.21223794109562003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100910.12866112360902932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115100.044.6201171
GTATCAA202150.039.830281
CAACGCA219250.034.918145
ATCAACG225200.033.9788063
TATCAAC254150.031.3487722
AACGCAG252750.031.1689626
GTACTAG67450.030.749641
GGGCACC76950.030.0495494
CTAACGC30500.029.6067243
TCTAACG30650.029.4623932
GTACATG301550.029.4147871
TCAACGC266450.029.2119224
TAACGCC31000.028.976744
TAGTACT91650.028.83914
TACATGG305200.028.5862142
CTGTGCG94600.028.3212349
ACATGGG300200.028.1061063
GCATCGT68450.028.0169378
ACGCAGA272300.028.0160967
CGCAGAG280000.027.1615458
Produced by FastQC (version 0.11.5)