FastQCFastQC Report
Thu 26 May 2016
ERR580255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7843084
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179910.22938680753642318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150920.19242430656104154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98220.12523135032086868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113650.045.2516941
GTATCAA202100.039.5662541
CAACGCA226400.033.5470965
ATCAACG232650.032.6864853
GTACTAG66800.031.6681731
TATCAAC255350.031.0697462
GGGCACC77950.030.57274694
AACGCAG263100.029.5992456
GTACATG299550.029.3780841
CTGTGCG89350.029.2982969
CTAACGC29050.029.283653
TAGTACT92300.028.8716344
TCTAACG30200.028.6359752
TACATGG303700.028.5685372
TCAACGC271400.028.348424
TAACGCC30100.028.1058064
ACATGGG299400.027.8009453
TAGGCAT82300.027.4685215
ACGCAGA283800.026.8110247
CGCCTAC32100.026.5004047
Produced by FastQC (version 0.11.5)