FastQCFastQC Report
Thu 26 May 2016
ERR580254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506673
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77310.17154561691074546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62120.13784004297627098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75200.045.206441
GTATCAA133700.040.1267551
CAACGCA149650.034.3713265
ATCAACG157300.032.7004663
TATCAAC167650.031.9451432
GTACTAG37900.031.5118831
GCATCGT38250.029.6060858
AACGCAG179750.029.5935446
TAGGCAT51900.028.3551985
GGGCACC38400.027.28934394
ACGCAGA188550.027.0397957
TCAACGC193750.026.9847744
CGCAGAG190600.026.6007528
GTGGTAT25050.025.9030691
CTGTGCG47200.025.6891779
TACCCCG4850.025.2050365
CTAGGCA58200.025.124254
TAGTACT48250.024.5560764
GCAGAGT206450.024.1075769
GTACATG240000.023.6273981
Produced by FastQC (version 0.11.5)