FastQCFastQC Report
Thu 26 May 2016
ERR580254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506673
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68100.15110925509794032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60950.13524389277855306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77450.044.6412281
GTATCAA134050.038.952411
CAACGCA153000.032.7132345
ATCAACG159700.031.3418373
TATCAAC169800.030.6127222
GTGGTAT27700.029.2888451
AACGCAG181300.028.7458346
GTACTAG38250.028.4861721
TCAACGC193250.026.2161754
TAGGCAT52200.026.1991835
ACGCAGA192600.025.9613047
TATTCTC21450.025.8535355
CGCAGAG195550.025.425478
TACCCCG5000.025.3780965
CGTTAAC15250.025.053521
GGGCACC40600.023.9609994
GTGATCG25800.023.8612258
CTGTGCG45200.023.704879
CTAGGCA57950.023.599874
GCAGAGT209450.023.4688859
Produced by FastQC (version 0.11.5)