FastQCFastQC Report
Thu 26 May 2016
ERR580253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8974012
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278600.3104520029614402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226210.25207231726456353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125080.13938024598139606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141650.051.4161721
GTATCAA255000.041.6156121
CAACGCA309550.033.5933885
ATCAACG312100.033.337513
TATCAAC324000.032.5943072
TCAACGC329350.031.6465914
AACGCAG335000.031.6029076
GTGGTAT59450.030.3699651
ACGCAGA373750.027.7487817
CGCAGAG384800.026.829678
TGGTATC65800.025.9392682
GTACATG336850.025.55741
TACATGG346300.024.2768332
GCAGAGT422400.023.9454739
GTACTAG40550.023.3061261
ACATGGG348500.023.1234973
CATGGGG266600.021.0377454
GAGTACT240100.020.81415412-13
CAGAGTA414000.020.15866510-11
AGAGTAC390200.020.14143410-11
Produced by FastQC (version 0.11.5)