FastQCFastQC Report
Thu 26 May 2016
ERR580253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8974012
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254140.28319552057652697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215950.24063930380302587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120620.1344103395448992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150650.052.1545831
GTATCAA267400.040.97081
ATCAACG325950.032.861813
CAACGCA328150.032.639865
TATCAAC340300.031.9714952
TCAACGC347850.030.8729274
AACGCAG353900.030.648546
GTGGTAT62000.027.1558321
ACGCAGA395200.027.0532237
CGCAGAG407200.026.1174858
GTACATG342850.024.2650411
GCAGAGT443550.023.7334049
TACATGG348150.023.1915992
TGGTATC70950.022.9853422
GTACTAG39350.022.8915541
ACATGGG353400.022.2232723
GAGTACT250650.021.58912712-13
CATGGGG268650.021.0106624
CAGAGTA434250.020.42031510-11
AGAGTAC411900.020.1250610-11
Produced by FastQC (version 0.11.5)