FastQCFastQC Report
Thu 26 May 2016
ERR580252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19464613
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494440.2540199489196112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372390.19131641610341804No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC281720.14473444707069183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243040.1248624876333272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC233400.041.9648694
GTGGTAT56150.040.8758851
GGTATCA344300.039.1913831
GTATCAA586650.038.1774941
GTACTAG275250.035.1482771
CAACGCA594050.035.132495
GCATCGT268550.033.795118
TAGGCAT316900.033.463315
ATCAACG625450.033.4178543
TATTCTC99750.033.284075
TACCCCG13750.032.491185
ACCTGGG49500.032.4000933
TATCAAC720350.031.0461252
AGGGCTA252650.030.1722955
TACCTGG54050.030.1079272
CTAGGCA367550.029.8140494
AACGCAG746000.029.668576
CTGTGCG358900.029.6495119
TATTAAG47000.029.3204212
TAGTACT369150.029.0095924
Produced by FastQC (version 0.11.5)