FastQCFastQC Report
Thu 26 May 2016
ERR580252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19464613
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429640.22072876558090318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339330.17433174756672531No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC288690.14831530429092016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229910.11811691298460442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC236950.043.60573694
GTGGTAT51450.040.110951
GGTATCA344800.039.551621
GTATCAA597450.038.77511
CAACGCA613350.034.980765
GTACTAG267600.034.729971
TACCCCG11250.034.2576945
ATCAACG640400.033.6064643
TAGGCAT312300.032.5221755
TATTCTC100050.032.0379035
TATCAAC744550.031.071912
AGGGCTA253650.030.870025
CTGTGCG367050.030.7175229
TAGTACT376250.029.942524
ACTGTGC383950.029.805978
AACGCAG775400.029.408426
CTAGGCA361600.029.2839454
TACTGTG408550.028.8048617
ACGCAGA750550.028.5098367
GTACTGT396450.028.474846
Produced by FastQC (version 0.11.5)