FastQCFastQC Report
Thu 26 May 2016
ERR580251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6821221
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136400.1999641999577495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106470.15608642499634595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90200.045.153161
GTATCAA151500.037.8414571
GTACTAG51300.033.278681
CAACGCA168800.031.8983635
GCATCGT49000.030.8844768
ATCAACG175100.030.8330573
GTACATG255050.030.0565451
TATCAAC190850.029.7441432
TACATGG260400.028.8075332
AACGCAG196000.028.2964426
ACATGGG256650.027.9807033
TAGGCAT63100.027.8726065
CTGTGCG70950.027.4252579
GTGGTAT31150.027.3273371
TAGTACT70050.027.1895084
CTAGGCA69400.026.6310064
TCAACGC204100.026.6129784
CTAACGC24300.026.1269283
TCTAACG25300.025.8392932
CTAGTAC77650.025.6794223
Produced by FastQC (version 0.11.5)