FastQCFastQC Report
Thu 26 May 2016
ERR580251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6821221
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124520.18254796318723585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100370.14714374449970175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69710.10219577990509324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94500.041.6367761
GTATCAA161150.039.7310681
CAACGCA180500.033.485355
ATCAACG189200.032.019883
TATCAAC207100.030.4564592
AACGCAG208650.029.8002436
TAGTACT74550.029.3152244
GGGCACC67200.029.24045294
CTGTGCG74850.028.8831399
CTAACGC21050.028.802193
GTACATG246700.028.544741
TCAACGC216850.028.2405344
CTAGTAC79600.027.5734863
TACATGG251000.027.5639292
TAACGCC22200.027.0984764
ACATGGG247900.026.9973663
ACGCAGA225250.026.8112267
TCTAACG22700.026.295712
GTACTGT87000.026.0919326
CGCAGAG232250.025.9424298
Produced by FastQC (version 0.11.5)