FastQCFastQC Report
Thu 26 May 2016
ERR580250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15602838
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516410.33097183986656786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401030.25702375426829405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258380.16559807901613796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA239300.049.8763241
GTATCAA437450.041.657081
CAACGCA500400.035.013225
ATCAACG514200.034.0771523
TATCAAC554700.032.5980832
AACGCAG563350.031.6630956
TCAACGC576450.030.6567964
GTACATG625950.030.261841
GTACTAG104850.030.0056631
TACATGG635350.029.1707292
ACATGGG622850.028.7367633
ACGCAGA615850.028.3842017
CGCAGAG635900.027.4667178
TAGTACT145000.026.6584844
GTGGTAT87600.026.5733681
CATGGGG488100.026.5137564
CTGTGCG146100.026.4126179
ATGGGGG374350.025.2604335
TAGGCAT133150.024.720855
GCAGAGT698000.024.7064749
Produced by FastQC (version 0.11.5)