FastQCFastQC Report
Thu 26 May 2016
ERR580250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15602838
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449320.28797325204555735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361640.23177834699046415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248570.1593107612858635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA241600.049.6751
GTATCAA430950.041.1024741
CAACGCA512950.033.242535
ATCAACG522800.032.6523863
TATCAAC561800.031.2314412
AACGCAG575300.030.2020246
TCAACGC586300.029.2838954
GTACATG611350.029.0122151
GTACTAG106700.028.5932981
TACATGG621500.028.080162
ACATGGG609050.027.6810823
ACGCAGA637400.026.765517
TAGTACT144550.026.6621284
CTGTGCG145400.026.3760369
CGCAGAG658900.025.9207638
CATGGGG476000.025.8895684
TAGGCAT134300.025.0925645
ATGGGGG362850.024.5720865
GGGCACC143050.024.15368894
GCAGAGT721550.023.4422449
Produced by FastQC (version 0.11.5)