FastQCFastQC Report
Thu 26 May 2016
ERR580249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8210723
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229510.27952471420604497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193980.2362520328599564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104570.1273578465623551No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125000.052.4014631
GTATCAA221000.041.0007441
CAACGCA263600.033.3712465
ATCAACG265000.033.1455573
TATCAAC276850.032.203182
TCAACGC279700.031.7055744
AACGCAG286350.031.1131786
ACGCAGA315750.027.8583347
CGCAGAG323500.027.0453598
GTGGTAT53400.027.0333231
GTACATG273350.025.854861
TACATGG274900.024.8197632
ACATGGG278350.023.8189283
TGGTATC59300.023.6301522
GCAGAGT364100.023.5049119
GTACTAG36200.022.9914841
CATGGGG212950.022.3012984
GAGTACT212400.021.89088412-13
ATGGGGG149900.020.8262215
CAGAGTA349750.020.72313910-11
Produced by FastQC (version 0.11.5)