FastQCFastQC Report
Thu 26 May 2016
ERR580249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8210723
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211250.2572855033594484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178200.2170332624788341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100240.1220842549432005No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123350.051.5020871
GTATCAA217850.039.920851
CAACGCA256750.033.0948835
ATCAACG258800.032.797613
TATCAAC276350.031.394272
TCAACGC273200.031.1717344
AACGCAG278400.030.9749496
ACGCAGA307250.027.592337
GTGGTAT52900.027.4594251
CGCAGAG318400.026.5227648
GTACATG264950.024.4223021
TGGTATC58650.023.5590342
GCAGAGT353550.023.5003979
TACATGG268450.023.4245282
ACATGGG269350.022.8233553
GAGTACT211900.021.45726612-13
CATGGGG208400.021.1087064
CAGAGTA345400.020.42848610-11
AGAGTAC323000.019.80835210-11
GTACTTT233050.019.37911614-15
Produced by FastQC (version 0.11.5)